Genetic architecture of common bean resistance to the root knot nematode Meloidogyne incognita: F2 population construction, linkage map, and QTL mapping

• Grant number: 22/04061-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2022
• End date: May 16, 2023
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Antonio Augusto Franco Garcia
• Grantee: Talissa de Oliveira Floriani Zimmermann de Souza
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 20/02755-2 - Common bean response to the root knot nematode Meloidogyne incognita: QLT mapping, transcriptional profile and functional studies of candidate resistance genes, AP.TEM

Abstract

Attacks by nematodes are among the main phytosanitary problems that affect common bean (Phaseolus vulgaris L.) crops. The production of P. vulgaris in the world can be challenging mainly due to the attack of the root-knot nematode (Meloidogyne incognita). Alternatives to control nematodes involve practices that are not viable in the field, and one of the most efficient ways is the development of new cultivars. Despite advances in genomics, little has been done to contribute to the understanding of the mechanisms involved in the common bean response to root-knot nematodes. In this scenario, the current project intends to continue the efforts in partnership with the Bean Improvement Program of the IAC and the Genetics Department of ESALQ, explicitly concerning the genetic architecture of the trait involved in the bean response root-knot nematode. This current project will be carried out within the scope of thematic project number 2020/02755-2, and the present proposal is in line with the ongoing project. A contrasting F2 population will be used to the resistance of M. incognita, derived from the cross between the parents PUEBLA-152 CIAT and JAMAPACNF1761. The population will be genotyped using the GBS (Genotyping by Sequencing) methodology to increase the number and exploration of single nucleotide polymorphisms (SNPs). The software OneMap will be used in the building of the genetic linkage map. With the help of the r/qtl software, the methodology for mapping multiple intervals (Multiple Interval Mapping; MIM) will be implemented to build the QTL mapping. Based on this project's results, it is expected: to understand the genetic architecture of nematode resistance; identify the number and positions of QTLs, their effects, and interactions between locus (epistasis). The present work will allow us to study the genetic architecture of nematode resistance, contributing to the development of selection strategies assisted by molecular markers and eventually strategies for better identification of genes involved in genetic control. In addition, the same characteristics were used in other populations of the same project; the results obtained here will be used as a form of validation of the identified genomic regions.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Talissa de Oliveira Floriani Zimmermann de. Genetic architecture of root-knot nematode resistance in common beans. 2023. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.