Phytonematodes are among the major phytosanitary problems on common bean (Phaseolus vulgaris), especially the root-knot nematodes (Meloidogyne spp.), perhaps the most widespread agricultural pest, and particularly the specie M. incognita, one of the most economically damaging pathogens. The control of these nematodes involves unfeasible agronomic practices, emphasizing the need for the development of resistant/tolerant cultivars, which are scarce for beans. Despite the availability of powerful genetic and molecular techniques, little was done to improve the understanding of the mechanisms involved in the common bean response to root-knot nematodes. In this context, the present project aims to detect, through genome-wide associative studies (GWAS), genomic regions associated with the common bean response to M. incognita, as well as identify candidate genes in RNA-Seq libraries (SANTINI, 2015) and by qRT-PCR. For the GWAS, a panel composed of 180 genotypes derived from a core collection of the IAC germplasm will be used. This panel was previously genotyped by the GBS approach, which allowed the identification of 82,680 SNPs (DINIZ, 2016). Once we map the associated genomic regions, RNA-Seq libraries obtained at four and 10 days after inoculation (SANTINI et al., 2015) will be used to verify the expression and select putative candidate genes. The expression of these genes will be confirmed by qRT-PCR, in genotypes presenting extreme responses. We expect to discover SNPs for genotype discrimination according to the response to M. incognita, contributing to selection process in common bean breeding programs, as well as identify putative candidate genes that may be validated in functional analyzes and eventually used for genome editing.
News published in Agência FAPESP Newsletter about the scholarship: