Genetic mapping reveals complex architecture and candidate genes involved in common bean response to Meloidogyne incognita infection

Author(s)

Giordani, Willian ^[1] ; Gama, Henrique Castro ^[1] ; Chiorato, Alisson Fernando ^[2] ; Rodrigues Marques, Joao Paulo ^[1] ; Huo, Heqiang ^[3] ; Benchimol-Reis, Luciana Lasry ^[2] ; Aranha Camargo, Luis Eduardo ^[1] ; Franco Garcia, Antonio Augusto ^[1] ; Carneiro Vieira, Maria Lucia ^[1]

Affiliation

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, BR-13418900 Sao Paulo - Brazil

^[2] Agron Inst Campinas, Grains & Fibers Ctr, BR-13075630 Campinas, SP - Brazil

^[3] Univ Florida, Midflorida Res & Educ Ctr, Apopka, FL 32703 - USA

• Document type: Journal article
• Source: PLANT GENOME; NOV 2021.
• Web of Science Citations: 0

Abstract

Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are among the most damaging and prevalent agricultural pathogens due to their ability to infect roots of almost all crops. The best strategy for their control is through the use of resistant cultivars. However, laborious phenotyping procedures make it difficult to assess nematode resistance in breeding programs. For common bean, this task is especially challenging because little has been done to discover resistance genes or markers to assist selection. We performed genome-wide association studies and quantitative trait loci mapping to explore the genetic architecture and genomic regions underlying the resistance to M. incognita and to identify candidate resistance genes. Phenotypic data were collected by a high-throughput assay, and the number of egg masses and the root-galling index were evaluated. Complex genetic architecture and independent genomic regions were associated with each trait. Single nucleotide polymorphisms on chromosomes Pv06, Pv07, Pv08, and Pv11 were associated with the number of egg masses, and SNPs on Pv01, Pv02, Pv05, and Pv10 were associated with root-galling. A total of 216 candidate genes were identified, including 14 resistance gene analogs and five differentially expressed in a previous RNA sequencing analysis. Histochemical analysis indicated that reactive oxygen species might play a role in the resistance response. Our findings open new perspectives to improve selection efficiency for RKN resistance, and the candidate genes are valuable targets for functional investigation and gene editing approaches. (AU)

• FAPESP's process: 20/02755-2 - Common bean response to the root knot nematode Meloidogyne incognita: QLT mapping, transcriptional profile and functional studies of candidate resistance genes
• Grantee: Maria Lúcia Carneiro Vieira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/09069-7 - Mapping of genomic regions and identification of putative candidate genes associated with common bean response to root-knot nematode (Meloidogine incognita)
• Grantee: Willian Giordani
• Support Opportunities: Scholarships in Brazil - Doctorate