Microspectrophotometry applied to the study of lignocellulose recalcitrance

• Grant number: 11/50535-2
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2011
• End date: June 30, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Andre Luis Ferraz
• Grantee: Andre Luis Ferraz
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

The aim of this project is to study the topochemical distribution of lignin and hydroxycinnamic acids in the cell walls of lignocelulosic materials. Modem methods will be developed to determine lignin structural characteristics and localization in cell walls using UV - microspectrophotometric techniques. The technique is based on the use of an especially designed optical microscope able to work in the UV spectral wavelengths (Zeiss/J&M). The microscopic technique will also permit to map lignin and hydroxycinnamic acids in pre-defined areas of the cellular complex by means of an automatic scanning stage device. The lignocellulosic materials to be studied include several sugar cane samples selected to cover commercial varieties and experimental hybrids containing varied concentrations of lignin and extractives. Samples with reduced lignin contents will be also prepared by selective delignification procedures. In all cases, the samples will be digested by a mixture of commercial cellulases aiming to correlate the recalcitrance of sugar cane cell walls with their lignin and hydroxycinnamic acid contents as well as their topochemical distribution. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, FERNANDA M.; FONSECA, MARIANA B.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Anatomic and Ultrastructural Characteristics of Different Regions of Sugar Cane Internodes Which Affect Their Response to Alkaline Sulfite Pretreatment and Material Recalcitrance. ENERGY & FUELS, v. 30, n. 2, p. 1078-1084, FEB 2016. (14/06923-6, 11/50535-2, 08/56256-5)

GRACIELA AGUAYO, MARIA; FERRAZ, ANDRE; ELISSETCHE, JUAN PEDRO; MASARIN, FERNANDO; MENDONCA, REGIS TEIXEIRA. Lignin chemistry and topochemistry during kraft delignification of Eucalyptus globulus genotypes with contrasting pulpwood characteristics. HOLZFORSCHUNG, v. 68, n. 6, p. 623-629, AUG 2014. (11/50535-2)

VARNAI, ANIKO; COSTA, THALES H. F.; FAULDS, CRAIG B.; MILAGRES, ADRIANE M. F.; SIIKA-AHO, MATTI; FERRAZ, ANDRE. Effects of enzymatic removal of plant cell wall acylation (acetylation, p-coumaroylation, and feruloylation) on accessibility of cellulose and xylan in natural (non-pretreated) sugar cane fractions. BIOTECHNOLOGY FOR BIOFUELS, v. 7, OCT 15 2014. (11/50535-2, 08/56256-5)

COSTA, THALES H. F.; MASARIN, FERNANDO; BONIFACIO, TAINA O.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. The enzymatic recalcitrance of internodes of sugar cane hybrids with contrasting lignin contents. INDUSTRIAL CROPS AND PRODUCTS, v. 51, p. 202-211, NOV 2013. (11/50535-2, 08/56256-5)

MASARIN, FERNANDO; NORAMBUENA, MARCELA; RAMIRES, HELOISA O. R.; DEMUNER, BRAZ J.; PAVAN, PAULO C.; FERRAZ, ANDRE. Manganese peroxidase and biomimetic systems applied to in vitro lignin degradation in Eucalyptus grandis milled wood and kraft pulps. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 91, n. 5, p. 1422-1430, MAY 2016. (11/50535-2)

COSTA, THALES H. F.; VEGA-SANCHEZ, MIGUEL E.; MILAGRES, ADRIANE M. F.; SCHELLER, HENRIK V.; FERRAZ, ANDRE. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 4 2016. (14/06923-6, 11/50535-2, 08/56256-5)

SOUSA, AURIZANGELA O.; CAMILLO, LUCIANA R.; ASSIS, ELZA THAYNARA C. M.; LIMA, NATHALIA S.; SILVA, GENILSON O.; KIRCH, ROCHELE P.; SILVA, DELMIRA C.; FERRAZ, ANDRE; PASQUALI, GIANCARLO; COSTA, MARCIO G. C.. EgPHI-1, aPHOSPHATE-INDUCED-1gene fromEucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties. PLANTA, v. 252, n. 3, SEP 2 2020. (11/50535-2)

MENDES, FERNANDA M.; HEIKKILA, ELINA; FONSECA, MARIANA B.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE; FARDIM, PEDRO. Topochemical characterization of sugar cane pretreated with alkaline sulfite. INDUSTRIAL CROPS AND PRODUCTS, v. 69, p. 60-67, JUL 2015. (11/08826-0, 11/50535-2, 08/56256-5)