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Expression of the cytoskeletal proteins actin and tubulin in osteogenic cells cultured on bioactive glass-based surfaces

Grant number: 11/01852-5
Support type:Regular Research Grants
Duration: September 01, 2011 - August 31, 2013
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Paulo Tambasco de Oliveira
Grantee:Paulo Tambasco de Oliveira
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Adalberto Luiz Rosa

Abstract

The implantation of bioactive glasses or glass-ceramics represents an important therapeutic strategy aiming to promote bone matrix formation in critical-sized bone defects. When a bioactive glass or glass-ceramic material interacts with biological fluids, changes in surface chemistry and surface topography take place, affecting the interactions of cells with the material surface, resulting in a reduction in cell spreading and altering the labeling pattern for cytoskeletal proteins and integrins. The present study aims to evaluate whether changes in the labeling pattern for actin and tubulin, which take place in osteogenic cells directly interacting with either Bioglass® 45S5 or Biosilicate® glass-ceramic, are due to a reduction in mRNA levels and/or in protein expression. At days 3 and 7 of primary osteogenic cell cultures, actin and tubulin mRNA levels and protein expression will be determined by means of Real-time PCR and enzyme immunoassay (ELISA), respectively. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, CAROLINA SCANAVEZ; FERRAZ, EMANUELA PRADO; SPINOLA DE CASTRO-RAUCCI, LARISSA MOREIRA; TEIXEIRA, LUCAS NOVAES; AMARU MAXIMIANO, WILLIAM MARCATTI; ROSA, ADALBERTO LUIZ; DE OLIVEIRA, PAULO TAMBASCO. Changes in actin and tubulin expression in osteogenic cells cultured on bioactive glass-based surfaces. MICROSCOPY RESEARCH AND TECHNIQUE, v. 78, n. 11, p. 1046-1053, . (11/01852-5)

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