Comparative genomics of species of fruit flies from the group fraterculus. II. The next generation

• Grant number: 10/20455-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2011
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Reinaldo Otávio Alvarenga Alves de Brito
• Grantee: Reinaldo Otávio Alvarenga Alves de Brito
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Vanessa Regina Gonçalves
• Associated research grant(s): 13/50202-9 - Comparison of intra- and inter-specific genetic architecture of speciation in South-American fruit flies, AP.R

Abstract

Even though the South American fruit flies Anastrepha fraterculus and A. obliqua are some of the greateste threats to the Brazilian fruiticulture, we still know very little of its biology and genetics. The existence of genetic maps and new genetic markers in these species are instrumental not only to establishing species-specific markers but also to foster evolutionary genomic studies that would increase the knowledge on these species. In a previous grant, we used ESTs (Expressed Sequenced Tags) data from reproductive tissues to isolate 100 SNPs (Single Nucleotide Polymorphisms) for these species and establish the first linkage map for A. obliqua. These data are important not only for its theoretical contributions on the evolutionary genomics processes that affected these species, but also for its practical application in quantitative strategies used to identify economically important genes. This project seeks to expand the EST analysis two-way, by the increase in the screening of the previously established cDNA libraries, but also by the use of modern next-gen sequencing methodologies. The use of these new methodologies, which are cheaper and more efficient, we want to isolate at least 5000 new SNPs, a number significantly higher than previously identified, 500 of which will be used to generate a linkage map with higher resolution for these species. The comparative genomics analyses of these high resolution maps will be used to help identify genes involved in the differentiation of these species and also in evolutionary genomics studies. In order to do so, we will also perform real time PCR to investigate potential differential expression between species and tissues for those candidate genes potentially involved in the species differentiation. The identification of these genes will be extremely useful for speciation studies but particularly should enable the establishment of better tools to control these important agricultural pests. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, JANAINA LIMA; SOBRINHO-JUNIOR, IDERVAL SILVA; CHAHAD-EHLERS, SAMIRA; DE BRITO, REINALDO ALVES. Evolutionary coincidence of adaptive changes in exuperantia and the emergence of bicoid in Cyclorrhapha (Diptera). DEVELOPMENT GENES AND EVOLUTION, v. 227, n. 5, p. 355-365, SEP 2017. (10/20455-4)

REZENDE, VICTOR BORGES; CONGRAINS, CARLOS; LIMA, ANDRE LUIS A.; CAMPANINI, EMELINE BONI; NAKAMURA, ALINE MINALI; DE OLIVEIRA, JANAINA LIMA; CHAHAD-EHLERS, SAMIRA; SOBRINHO JUNIOR, IDERVAL; DE BRITO, REINALDO ALVES. Head Transcriptomes of Two Closely Related Species of Fruit Flies of the Anastrepha fraterculus Group Reveals Divergent Genes in Species with Extensive Gene Flow. G3-GENES, GENOMES, GENETICS, v. 6, n. 10, p. 3283-3295, OCT 2016. (10/20455-4)

CAMPANINI, EMELINE BONI; CONGRAINS, CARLOS; TORRES, FELIPE RAFAEL; DE BRITO, REINALDO ALVES. . Odorant-binding proteins expression patterns in recently diverged species of Anastrepha fruit flies. SCIENTIFIC REPORTS, v. 7, MAY 19 2017. (12/17160-8, 10/20455-4)

CHAVES REGO, NANCY DE FATIMA; CHAHAD-EHLERS, SAMIRA; CAMPANINI, EMELINE BONI; TORRES, FELIPE RAFAEL; DE BRITO, REINALDO ALVES. VRILLE shows high divergence among Higher Diptera flies but may retain role as transcriptional repressor of clock. JOURNAL OF INSECT PHYSIOLOGY, v. 133, AUG-SEP 2021. (18/06611-5, 10/20455-4)

CONGRAINS, CARLOS; CAMPANINI, EMELINE B.; TORRES, FELIPE R.; REZENDE, VICTOR B.; NAKAMURA, ALINE M.; DE OLIVEIRA, JANAINA L.; LIMA, ANDRE L. A.; CHAHAD-EHLERS, SAMIRA; SOBRINHO, JR., IDERVAL S.; DE BRITO, REINALDO A.. Evidence of Adaptive Evolution and Relaxed Constraints in Sex-Biased Genes of South American and West Indies Fruit Flies (Diptera: Tephritidae). GENOME BIOLOGY AND EVOLUTION, v. 10, n. 1, p. 380-395, JAN 2018. (12/17160-8, 10/20455-4)

CAMPANINI, EMELINE BONI; DE BRITO, REINALDO ALVES. Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies. BMC Evolutionary Biology, v. 16, OCT 7 2016. (12/17160-8, 10/20455-4)

CHAHAD-EHLERS, S.; ARTHUR, L. P.; LIMA, A. L. A.; GESTO, J. S. M.; TORRES, F. R.; PEIXOTO, A. A.; DE BRITO, R. A.. Expanding the view of Clock and cycle gene evolution in Diptera. Insect Molecular Biology, v. 26, n. 3, p. 317-331, JUN 2017. (10/20455-4)

GONCALVES, V. R.; SOBRINHO, JR., I. S.; MALAGO-, JR., W.; HENRIQUE-SILVA, F.; DE BRITO, R. A.. Transcriptome analysis of female reproductive tissues of Anastrepha obliqua and molecular evolution of eggshell proteins in the fraterculus group. Insect Molecular Biology, v. 22, n. 5, p. 551-561, OCT 2013. (10/20455-4)

NAKAMURA, ALINE MINALI; CHAHAD-EHLERS, SAMIRA; LIMA, ANDRE LUIS A.; TANIGUTI, CRISTIANE HAYUMI; SOBRINHO, JR., IDERVAL; TORRES, FELIPE RAFAEL; DE BRITO, REINALDO ALVES. Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua. SCIENTIFIC REPORTS, v. 6, JAN 28 2016. (10/20455-4)

DIAZ, FERNANDO; WOLF, JASON; DE BRITO, REINALDO A.. Transcriptional Misexpression in Hybrids between Species Linked by Gene Flow Is Associated With Patterns of Sequence Divergence. GENOME BIOLOGY AND EVOLUTION, v. 15, n. 5, p. 19-pg., 2023-05-05. (14/50940-2, 18/06611-5, 10/20455-4)

DIAZ, FERNANDO; LIMA, ANDRE LUIS A.; NAKAMURA, ALINE M.; FERNANDES, FERNANDA; SOBRINHO, JR., IDERVAL; DE BRITO, REINALDO A.. Evidence for Introgression Among Three Species of the Anastrepha fraterculus Group, a Radiating Species Complex of Fruit Flies. FRONTIERS IN GENETICS, v. 9, SEP 10 2018. (10/20455-4)