Molecular cloning of GnRH of Astyanax altiparanae and its effect in vivo

• Grant number: 11/09567-8
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2011
• End date: February 28, 2014
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maria Ines Borella
• Grantee: Maria Ines Borella
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Silvana Auxiliadora Bordin da Silva

Abstract

The study on fish reproduction gained importance in last years due the possibility of manipulation of the reproductive process, especially in endangered species. During this process, Kisspeptin regulated hypothalamic neurons produce GnRH, which stimulates the pituitary cells to release FSH and LH, which promote gonadal maturation. The GnRH, beyond the reproductive function, participates of the neuromodulation of sexual behavior. Thus, the knowledge of the specific hypophysiotropic isoform of this molecule for each species can be crucial for efficient reproduction. Currently there are 25 isoforms of GnRH described among vertebrates and invertebrates, two or three of these isoforms normally found in a single species. In addition, the GnRH is considered a marker for studies of evolution, since the genes encoding the GnRH in vertebrates have the same architecture. Thus, we intend to make the molecular cloning of the GnRH of lambari Astyanax altiparanae, a South American species with relevant importance, and, with the injection of the obtained peptide, analyze the proteic expression of LH and the synthesis of gonadal steroids. Finally, it may be an attempt to improve the conditions of spawning in captivity of the same order species (Characiforms), which represents the majority of the cultivated species in Brazil, since most of these animals kept in captivity displays difficulty in reproduce without hormonal induction. So, the research is important in academic area because of the molecular cloning of the GnRH gene in Characiforms, and in ecological and economic area because of the future improvement in the practice of fish farming that the results of this research can bring. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

CASSEL, MONICA; CHEHADE, CHAYRRA; BRANCO, GIOVANA SOUZA; CANEPPELE, DANILO; ROMAGOSA, ELIZABETH; BORELLA, MARIA INES. Ovarian development and the reproductive profile of Astyanax altiparanae (Teleostei, Characidae) over one year: Applications in fish farming. Theriogenology, v. 98, p. 1-15, AUG 2017. (12/24434-7, 14/23451-0, 11/09567-8)

CHEHADE, CHAYRRA; CASSEL, MONICA; BORELLA, MARIA INES. Induced reproduction in a migratory teleost species by water level drawdown. Neotropical Ichthyology, v. 13, n. 1, p. 205-211, JAN-MAR 2015. (10/17586-0, 11/09567-8)

CHEHADE, CHAYRRA; AMARAL, FERNANDA G.; BRANCO, GIOVANA S.; CASSEL, MONICA; DE JESUS, LAZARO W. O.; COSTA, FABIANO G.; BORDIN, SILVANA A.; MOREIRA, RENATA G.; BORELLA, I, MARIA. Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction. Molecular Reproduction and Development, v. 87, n. 6, MAY 2020. (10/17586-0, 11/09567-8)