Application of thromboelastometry as a clinical tool for hemostatic evaluation in poultry submitted to vitamin K deficiency induced by sulfonamide administration

• Grant number: 11/12972-1
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2011 - October 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Regina Kiomi Takahira
• Grantee: Regina Kiomi Takahira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Although little known, avian hemostasis is becoming more relevant in clinic and productive areas, where hemorrhagic disorders cause economic lost due to an increase in carcasses' condemnations and in flock mortality. The intoxication with sulfaquinoxalin, which cause vitamin K antagonism leading to the hemorrhagic syndrome in poultry, is one of the most important condition among avian hemorrhagic disorders. Although the physiologic process of avian hemostasis is similar to mammals, many results discrepancy are seen in literature, and may occur, at least in part, due to the use of non homologous reagents. Avian thrombocytes have analogous function to mammalian platelets, but are estructurally different and other functions, such as its phagocytic activity has also been investigated. Recent studies with emphasis in platelets and cells that release tissue factor demonstrate that they contribute in whole coagulation process. Commercial hemostatic tests currently used in the laboratory routine evaluate only isolated phases or components of coagulation and don't incorporate cellular elements. The thromboelastometry is the better test to reflect cellular participation in coagulation, providing a complete evaluation of hemostatic system from cinetics of clotting till fibrinolysis. The present study will evaluate possible hemorragic disorders caused by sulfaquinoxalin and evaluate the use of thromboelastometry in studies of avian hemostasis. Forty healthy poultry, Cobb lineage, will be divided in two groups: Group 1 or control group with 20 poultry and no sulfaquinoxalin administration, and Group 2 with 20 poultry treated with sulfaquinoxalin. Both groups will be evaluated for hemostatic and hematological profile during the experimental period. (AU)