| Grant number: | 11/05876-6 |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| Start date: | November 01, 2011 |
| End date: | October 31, 2015 |
| Field of knowledge: | Biological Sciences - Physiology - General Physiology |
| Principal Investigator: | Alice Cristina Rodrigues |
| Grantee: | Alice Cristina Rodrigues |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated scholarship(s): | 14/22046-5 - Effect of pioglitazone on the expression of skeletal muscle microRNAs in hyperlipidic diet-induced obesity,
BP.IC 13/19787-0 - The role of microRNAs (miR-1, miR-133, miR-206, miR-208b, miR-499 e miR-223)in skeletal muscle of C57BL/6 mouse during insulin resistance, BP.MS 11/20453-4 - Role of microRNAs in the development of insulin resistance, BP.JP |
Abstract
A clinically relevant question is whether microRNA (miR or miRNA) levels are capable of assessing the probability of diabetes mellitus type 2 (DM2) manifestations in patients with insulin resistance. The aim of this study is to investigate which microRNAs regulate the genes involved with insulin resistance after high fat diet and whether miRNAs could be biomarkers for DM2 manifestation and/or progression. Male C57BL/6 mice will receive a balanced or high-fat diet during 4, 8 or 12 weeks (weeks 0 to 12). In the week 10, a group of mice will continue receiving balanced or high-fat diet supplemented with ezetimibe, fenofibrate or rosiglitazone. At the end of the treatment, the following assessments will be performed: insulin sensitivity; plasma parameters determinations, such as total cholesterol and fractions, triglycerides, hepatic and enzymes, glucose and insulin; analyses and identification of miRNAs in plasma, liver, adipocyte and skeletal muscle; mRNA expression of identified miRNA target genes and functional analysis of miRNAs. A better understanding of the mechanisms involved in miRNAs liberation in circulation and in their regulation of genes associated with insulin resistance will have impact on the elucidation of the issues related to diabetes progression, as well as the development of new therapies that targets miRNAs for therapeutic intervention. (AU)
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