Populational characterization of orchid bees (Apidae, Euglossini) from São Paulo State using geometric morphometrics of wings, mitochondrial DNA variability and cuticular hydrocarbons

• Grant number: 11/07857-9
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
• Duration: December 01, 2011 - November 30, 2015
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Tiago Mauricio Francoy
• Grantee: Tiago Mauricio Francoy
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Carlos Alberto Garofalo ; Fernando de Faria Franco ; Marco Antonio Del Lama
• Associated scholarship(s): 13/02158-0 - Altitudinal and geographic effects on the genetic variability of orchid bees (Apidae, Euglossini) populations of the State of São Paulo, BP.DR

Abstract

The current pollinators decline, especially bee populations, is a serious global problem which demands efforts to monitor its biodiversity, conservation and sustainable use. Given the current status of the taxonomic impediment faced nowadays, a key component is the development and application of auxiliary tools that allow us to assess intra and inter-specific variation. The characterization of mitochondrial DNA variability through sequencing of determined regions allow the evaluation of the evolutionary history of the groups and also the haplotypes variation, generating essential data for its conservation. Another very promising method is the usage of computational tools allied to geometric morphometrics analysis of the forewings' venation patterns. Recent data show the effectiveness of the technique in discriminating species, evaluating genetic variability of groups and in differentiating ecotypes. Thus, this project aims the application of the cited techniques in some euglossine bee populations, from several localities in São Paulo state, so we can evaluate the populational variability of the studied groups. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, FABIANA S.; REALI COSTA, ANNA H.; TRUZZI, FLAVIO S.; SILVA, FELIPE L.; SANTOS, SHEILA L.; FRANCOY, TIAGO M.; SARAIVA, ANTONIO M.. A reference process for automating bee species identification based on wing images and digital image processing. ECOLOGICAL INFORMATICS, v. 24, p. 248-260, NOV 2014. (11/19280-8, 11/07857-9)

JAFFE, RODOLFO; PIOKER-HARA, FABIANA C.; DOS SANTOS, CHARLES F.; SANTIAGO, LEANDRO R.; ALVES, DENISE A.; KLEINERT, ASTRID DE M. P.; FRANCOY, TIAGO M.; ARIAS, MARIA C.; IMPERATRIZ-FONSECA, VERA L.. Monogamy in large bee societies: a stingless paradox. NATURWISSENSCHAFTEN, v. 101, n. 3, p. 261-264, MAR 2014. (10/19717-4, 12/13200-5, 11/07857-9)

GALASCHI-TEIXEIRA, JULIANA S.; FALCON, TIAGO; FERREIRA-CALIMAN, MARIA JULIANA; WITTER, SIDIA; FRANCOY, TIAGO MAURICIO. Morphological, chemical, and molecular analyses differentiate populations of the subterranean nesting stingless bee Mourella caerulea (Apidae: Meliponini). Apidologie, v. 49, n. 3, p. 367-377, JUN 2018. (11/02434-2, 11/07857-9)

COSTA, C. P.; FRANCOY, T. M.. The Impact of Different Phytophysiognomies on the Composition of Orchid Bee Communities (Hymenoptera: Apidae: Euglossini) in the Atlantic Forest in Brazil. Annals of the Entomological Society of America, v. 110, n. 3, p. 255-262, MAY 2017. (13/02158-0, 11/07857-9)

BONATTI, VANESSA; PAULINO SIMOES, ZILA LUZ; FRANCO, FERNANDO FARIA; FRANCOY, TIAGO MAURICIO. Evidence of at least two evolutionary lineages in Melipona subnitida (Apidae, Meliponini) suggested by mtDNA variability and geometric morphometrics of forewings. NATURWISSENSCHAFTEN, v. 101, n. 1, p. 17-24, JAN 2014. (11/07857-9)

MORETTI, C. J.; COSTA, C. P.; FRANCOY, T. M.. Wing morphometrics reveals the migration patterns of Africanized honey bees in Northeast Brazil. Sociobiology, v. 65, n. 4, SI, p. 679-685, OCT 2018. (11/07857-9)

GRASSI-SELLA, MARINA LOPES; GAROFALO, CARLOS ALBERTO; FRANCOY, TIAGO MAURICIO. Morphological similarity of widely separated populations of two Euglossini (Hymenoptera; Apidae) species based on geometric morphometrics of wings. Apidologie, v. 49, n. 2, p. 151-161, APR 2018. (11/07857-9)

FRANCOY, T. M.; BONATTI, V.; VIRAKTAMATH, S.; RAJANKAR, B. R.. Wing morphometrics indicates the existence of two distinct phenotypic clusters within population of Tetragonula iridipennis (Apidae: Meliponini) from India. Insectes Sociaux, v. 63, n. 1, p. 109-115, FEB 2016. (13/20358-7, 11/07857-9)

FRANCOY, T. M.; GONCALVES, L. S.; DE JONG, D.. Rapid morphological changes in populations of hybrids between Africanized and European honey bees. Genetics and Molecular Research, v. 11, n. 3, p. 3349-3356, 2012. (11/07857-9)

FRANCOY, TIAGO MAURICIO; FRANCO, FERNANDO DE FARIA; ROUBIK, DAVID W.. Integrated landmark and outline-based morphometric methods efficiently distinguish species of Euglossa (Hymenoptera, Apidae, Euglossini). Apidologie, v. 43, n. 6, p. 609-617, NOV 2012. (11/07857-9)

COSTA, CLAUDINEIA PEREIRA; MACHADO, CLYCIE APARECIDA DA SILVA; FRANCOY, TIAGO MAURICIO. Assessment of genetic diversity and population structure of Eulaema nigrita (Hymenoptera: Apidae: Euglossini) as a factor of habitat type in Brazilian Atlantic forest fragments. CANADIAN ENTOMOLOGIST, v. 153, n. 4, p. 446-460, AUG 2021. (13/02158-0, 11/07857-9)

HENRIQUES, DORA; CHAVEZ-GALARZA, JULIO; TEIXEIRA, S. G. JULIANA; FERREIRA, HELENA; NEVES, J. CATIA; FRANCOY, TIAGO M.; PINTO, M. ALICE. Wing Geometric Morphometrics of Workers and Drones and Single Nucleotide Polymorphisms Provide Similar Genetic Structure in the Iberian Honey Bee (Apis mellifera iberiensis). INSECTS, v. 11, n. 2, FEB 2020. (11/02434-2, 11/07857-9)

MACHADO, C. A. S.; COSTA, C. P.; FRANCOY, T. M.. Different Physiognomies and the Structure of Euglossini Bee (Hymenoptera: Apidae) Communities. Sociobiology, v. 65, n. 3, p. 471-481, SEP 2018. (13/02158-0, 11/07857-9)

DA SILVA, FELIPE LENO; GRASSI SELLA, MARINA LOPES; FRANCOY, TIAGO MAURICIO; REALI COSTA, ANNA HELENA. Evaluating classification and feature selection techniques for honeybee subspecies identification using wing images. COMPUTERS AND ELECTRONICS IN AGRICULTURE, v. 114, p. 68-77, JUN 2015. (11/07857-9)

DE SOUZA, DAIANA A.; WANG, YING; KAFTANOGLU, OSMAN; DE JONG, DAVID; AMDAM, GRO V.; GONCALVES, LIONEL S.; FRANCOY, TIAGO M.. Morphometric Identification of Queens, Workers and Intermediates in In Vitro Reared Honey Bees (Apis mellifera). PLoS One, v. 10, n. 4, APR 20 2015. (11/07857-9)

COSTA, CLAUDINEIA PEREIRA; SILVA MACHADO, CLYCIE APARECIDA; SOUZA SANTIAGO, WELLYNGTON MATHEUS; DALLACQUA, RODRIGO PIRES; GAROFALO, CARLOS ALBERTO; FRANCOY, TIAGO MAURICIO. Biome variation, not distance between populations, explains morphological variability in the orchid beeEulaema nigrita(Hymenoptera, Apidae, Euglossini). Apidologie, v. 51, n. 6, JUL 2020. (11/07857-9, 13/02158-0)