Optimization of diagnosis of genetically modified soybean and mayze by Real Time PCR

• Grant number: 11/12232-8
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2012
• End date: December 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Haiko Enok Sawazaki
• Grantee: Haiko Enok Sawazaki
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

The detection and quantification of genetically modified organism (GMO) is required in almost all countries to which Brazil exports food. Currently in Brazil already there are genetically modified crops of cotton, soybean and corn approved for commercial production, with fifteen OGMs events for corn and five for soybeans. The official methods used throughout Europe are methods validated by the European Commission, responsible for European Union Reference Laboratory for GM food and feed. These methods use the technique of event-specific quantitative analysis and some diagnosis methods of soybean and corn events found in Brazil are in the JRC Reference Compendium of Methods for GMO analysis (JRC-ISO/FDIS). This study aims to establish diagnosis methods for corn and soybeans genetically modified, or optimize the official european method which utilize the Taqman Real Time PCR systems, by using the SYBR Green or Green BRYTTM (supposed to exhibit higher fluorescence in double strand DNA than SYBR) systems, in order to implement a method of lowest cost, but effective, which allows the request of ISO 17025 by the laboratory. (AU)