Detection of microorganisms in the saliva and self-ligating brackets by the checkerboard DNA-DNA hybridization: in vivo

• Grant number: 10/16757-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: January 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Mírian Aiko Nakane Matsumoto
• Grantee: Mírian Aiko Nakane Matsumoto
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Ana Zilda Nazar Bergamo ; Marcela Cristina Damião Andrucioli ; Paulo Nelson Filho

Abstract

The purpose of this study will be to evaluate in vivo by the Checkerboard DNA-DNA Hybridization technique : 1) increase of the bacterial contamination in the saliva before and after the orthodontic bonding; 2) to evaluate if the plaque accumulation is lesser in the metallic bracket or self-ligating bracket; 3) to evaluate if will be difference in the plaque accumulation between the self-ligating brackets with different drawings (In-Ovation, Dentsply, GAC and Smartclip, 3M-Unitek). 25 patients will participate of this study (12-25 years), which will receive metallic brackets and self-ligating brackets bonded to different premolars in a randomized manner, between the arcs upper/lower and the sides right and left. The contamination will be evaluated in the saliva after 30 and 60 days after bonding. The brackets contamination will be evaluated in the same time. The results will be analyzed by the statistical means of the Kruskal-Wallis test, Mann-Whitney and Dunn test. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAZAR BERGAMO, ANA ZILDA; NELSON-FILHO, PAULO; DO NASCIMENTO, CASSIO; VIANA CASARIN, RENATO CORREA; CASATI, MARCIO ZAFFALON; DAMIDO ANDRUCIOLI, MARCELA CRISTINA; KUCHLER, ERIKA CALVANO; LONGO, DANIELE LUCCA; BEZERRA DA SILVA, LEA ASSED; NAKANE MATSUMOTO, MIRIAN AIKO. Cytokine profile changes in gingival crevicular fluid after placement different brackets types. ARCHIVES OF ORAL BIOLOGY, v. 85, p. 79-83, JAN 2018. (10/16757-5)

NAZAR BERGAMO, ANA ZILDA; NAKANE MATSUMOTO, MIRIAN AIKO; DO NASCIMENTO, CASSIO; DAMIAO ANDRUCIOLI, MARCELA CRISTINA; ROMANO, PABLO LOURENCO; BEZERRA SILVA, RAQUEL ASSED; BEZERRA SILVA, LEA ASSED; NELSON-FILHO, PAULO. Microbial species associated with dental caries found in saliva and in situ after use of self-ligating and conventional brackets. Journal of Applied Oral Science, v. 27, 2019. (10/16757-5)

NAZAR BERGAMO, ANA ZILDA; NELSON-FILHO, PAULO; DAMIAO ANDRUCIOLI, MARCELA CRISTINA; DO NASCIMENTO, CASSIO; PEDRAZZI, VINICIUS; NAKANE MATSUMOTO, MIRIAN AIKO. Microbial complexes levels in conventional and self-ligating brackets. CLINICAL ORAL INVESTIGATIONS, v. 21, n. 4, p. 1037-1046, MAY 2017. (10/16757-5)

BERGAMO, ANA ZILDA NAZAR; CASARIN, RENATO CORREA VIANA; DO NASCIMENTO, CASSIO; MATSUMOTO, MIRIAN AIKO NAKANE; DE CARVALHO, FABRICIO KITAZONO; DA SILVA, RAQUEL ASSED BEZERRA; DA SILVA, LEA ASSED BEZERRA; NELSON-FILHO, PAULO. Self-ligating brackets exhibit accumulation of high levels of periodontopathogens in gingival crevicular fluid. ODONTOLOGY, v. 110, n. 3, p. 7-pg., 2022-01-17. (10/16757-5)