Detection of Escherichia coli Shiga toxin (STEC) and enteropathogenic (EPEC) in fish from fish farming in the region of Ribeirão Preto - SP

• Grant number: 11/15050-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: March 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Fernando Antonio de Avila
• Grantee: Fernando Antonio de Avila
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The expansion of aquaculture and increased consumption of fish meat make research in this area, especially if related to health and public health, a global trend and need. Although Escherichia coli is not a natural organism of the intestinal tract of fish, it is known that the microflora of these animals is directly linked to quality of water in which they live. Shigatoxigenic Escherichia coli (STEC) and enteropathogenic (EPEC) are pathogens of importance in public health from the potential transmission of enteric diseases to humans and the possible emergence of multiresistant isolates. Based on this, this project aims to detect by PCR, STEC and EPEC strains, and characterize virulence genes of isolates from the gut contents and muscle of fish and fish-farming in the water in which they are created. After isolation, the strains will be subjected to determination of serogroup and profile test for resistance to antibiotics. Also, it will be established an epidemiological link of the isolates by PFGE and phylogenetic analysis by PCR for Chua, and YjaA TspE4C2. The results of this study will be instrumental in helping to understand the epidemiology of E. coli bacteria in fish and to observe the ability of transmitting diseases to humans. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOZO, MARITA VEDOVELLI; BORGES, CLARISSA ARAUJO; BERALDO, LIVIA GERBASI; MALUTA, RENATO PARIZ; POLLO, ANDRESSA SOUZA; BORZI, MARIANA MONEZI; DOS SANTOS, LUIS FERNANDO; KARIYAWASAM, SUBHASHINIE; DE AVILA, FERNANDO ANTONIO. Shigatoxigenic and atypical enteropathogenic Escherichia coli in fish for human consumption. Brazilian Journal of Microbiology, v. 49, n. 4, p. 936-941, OCT-DEC 2018. (11/07358-2, 11/15050-8)

ARIEL EURIDES STELLA; MARIA CRISTINA DE OLIVEIRA; VERA LÚCIA DIAS DA SILVA FONTANA; RENATO PARIS MALUTA; CLARISSA ARAÚJO BORGES; FERNANDO ANTÔNIO DE ÁVILA. Padrões da caracterização e resistência a antimicrobianos de Escherichia coli isolada das fezes de frangos de corte saudáveis. Arq. Inst. Biol., v. 83, 2016-09-15. (08/00417-0, 11/15050-8)