Application of comprehensive bidimensional gas chromatography to the search of biomarkers for pathogen resistance on vegetables

• Grant number: 11/51896-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: February 28, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Agreement: CNPq - REPENSA
• Principal Investigator: Fabio Augusto
• Grantee: Fabio Augusto
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This project is inserted on the Brazilian National Research Network on Agrodiversity and Agricultural Sustainability (REPENSA) through the study and characterization of the metabolic profiles of vegetable, aiming the understanding of the biochemical mechanisms associated to the induction of plant resistance to pathogen agents and insects. We presented a proposal to develop analytical strategies for analysis of volatile and semi-volatile compounds from plants. For the identification and quantitation, conventional (GC) and Comprehensive Multidimensional Gas Chromatography (GCxGC) coupled to quadrupolar mass spectrometry (qMS) will be employed to study the metabolic profile of plants, especially eucalyptus. Methods for the analytical characterization of primary and secondary metabolites from vegetable species will be developed. Samples from healthy and pathogen fungi-infected plants obtained from eucalyptus commercial cultivars will be analyzed. Solid Phase Microextraction (SPME) will be used as sample preparation technique. It is expected that the results obtained help the understanding of the biochemical processes involved on plant defense mechanisms during pathogen-induced stress periods. Also, we hope that the analytical tools studied can be applicable to fast determination of pathogen-resistant eucalyptus cultivars. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELINATO DE SOUZA, JOAO R.; DIAS, FERNANDA F. G.; CALIMAN, JAQUELINE D.; AUGUSTO, FABIO; HANTAO, LEANDRO W.. Opportunities for green microextractions in comprehensive two-dimensional gas chromatography / mass spectrometry-based metabolomics - A review. Analytica Chimica Acta, v. 1040, p. 1-18, DEC 21 2018. (11/51896-9, 17/25490-1, 15/05059-9, 16/20547-2)

HANTAO, LEANDRO WANG; DE LIMA RIBEIRO, FABIANA ALVES; PASSADOR, MARTHA MARIA; FURTADO, EDSON LUIZ; POPPI, RONEI JESUS; GOZZO, FABIO CESAR; AUGUSTO, FABIO. Metabolic profiling by ultra-performance liquid chromatography-mass spectrometry and parallel factor analysis for the determination of disease biomarkers in Eucalyptus. METABOLOMICS, v. 10, n. 6, p. 1318-1325, DEC 2014. (11/51896-9)

LEANDRO W. HANTAO; BRUNA R. TOLEDO; FABIO AUGUSTO. FASES ESTACIONÁRIAS DE LÍQUIDOS IÔNICOS EM CROMATOGRAFIA GASOSA: FUNDAMENTOS, AVANÇOS RECENTES E PERSPECTIVAS. Química Nova, v. 39, n. 1, p. 81-93, 2016-01-00. (12/22375-3, 11/51896-9)