Evaluation of the effectiveness of the use of insecticide impregnated collar and subunit vaccine against canine visceral leishmaniasis

• Grant number: 11/21796-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2012
• End date: August 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Rodrigo Martins Soares
• Grantee: Rodrigo Martins Soares
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Fernando Ferreira

Abstract

This study aims to evaluate the effectiveness of a subunit vaccine against leishmaniasis in dogs as well as the use of a repellent insecticide-impregnated collar through a cohort study to be conducted in two regions, being one with a high transmission and the other with a low transmission of canine visceral leishmaniasis. To this end, we propose the construction of eight cohorts, four of them in the in area of high transmission and four in the area of low transmission. In each area there will be a cohort of vaccinated individuals, a cohort of individuals with a collar impregnated with repellent, a cohort of individuals vaccinated and with collar impregnated with repellent, and finally, a cohort of unvaccinated and without collars. Biological samples will be collected from animals of all cohorts in three occasions at the beginning of the experiment, six months after the start of the experiment and one year after the start of the experiment. Serum samples will be subjected to serodiagnosis by ELISA and IFAT, the whole blood samples for PCR and isolation of the agent, the lymph node puncture for PCR and isolation of the agent and the skin biopsy for PCR. The impact of control measures for VL in dogs will be measured by the ratio of odds of seroconversion and / or PCR positivity (CI 95%) among subjects exposed and not exposed to risk of acquiring the parasite. Exposure to risk means the absence of control measures (vaccination or collar). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, E. G.; SEVA, A. P.; FERREIRA, F.; NUNES, C. M.; KEID, L. B.; HIRAMOTO, R. M.; FERREIRA, H. L.; OLIVEIRA, T. M. F. S.; BIGOTTO, M. F. D.; GALVIS-OVALLOS, F.; et al. Serological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infected. EPIDEMIOLOGY AND INFECTION, v. 145, n. 12, p. 2436-2444, SEP 2017. (11/14892-5, 11/21796-2)

LOPES, E. G.; SEVA, A. P.; FERREIRA, F.; NUNES, C. M.; KEID, L. B.; HIRAMOTO, R. M.; FERREIRA, H. L.; OLIVEIRA, T. M. F. S.; OVALLOS, F. G.; GALATI, E. A. B.; et al. Vaccine effectiveness and use of collar impregnated with insecticide for reducing incidence of Leishmania infection in dogs in an endemic region for visceral leishmaniasis, in Brazil. EPIDEMIOLOGY AND INFECTION, v. 146, n. 3, p. 401-406, FEB 2018. (11/14892-5, 11/21796-2)