Time course of the inflammatory process in classical ballet dancers: leukocytes and systemic alterations

• Grant number: 11/21441-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Elaine Hatanaka Dermargos
• Grantee: Elaine Hatanaka Dermargos
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil

Abstract

The positions of classical ballet are anti-anatomical, fact that generates joint misalignments and inflammatory lesions on dancers. In general, systemic chronic inflammation alters the body homeostasis, resulting in changes in the number/function of leukocytes and decreasing the athletes' performance. Herein, we intend to investigate the time course of the inflammatory process (installation and resolution) in dancers after a ballet class. The dancers will be grouped according to the VO2max, experience of at least 5 years of dancing and frequency of training of at least 3 times a week. It will be evaluated in plasma or serum of dancers before, immediately after and 24 hours after a class: (a) pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-a), interleukin 6 (IL- 6), interleukin 8 (IL-8) and interleukin 1beta (IL-1 b), anti-inflammatory cytokines IL-ra, IL-10 and IL-4 (b) markers of muscle damage creatine Quinase (CK) and lactate dehydrogenase (LDH), (c) acute phase protein CRP and (d) immunoglobulin A (IgA) and M (IgM). In neutrophils we will analyze the following parameters: (e) cell death (viability assays, DNA fragmentation and externalization of phosphatidylserine), (f) production of EROS and (g) release of cytokines. Cytokines will be quantified by using enzyme immunoassay (ELISA) method, markers of injury will be quantified by spectrophotometry and leukocyte changes will be verified by flow cytometry. These data may help physiologists / therapists / teachers of classical ballet devise strategies for controlling processes that may result in infection and tissue injury, thus improving performance and rates of chronic lesions in dancers. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGES, LEANDRO DA SILVA; BORTOLON, JOSE RICARDO; SANTOS, VINICIUS CONEGLIAN; DE MOURA, NIVALDO RIBEIRO; DERMARGOS, ALEXANDRE; CURY-BOAVENTURA, MARIA FERNANDA; GORJAO, RENATA; PITHON-CURI, TANIA CRISTINA; HATANAKA, ELAINE. Chronic Inflammation and Neutrophil Activation as Possible Causes of Joint Diseases in Ballet Dancers. Mediators of Inflammation, 2014. (11/21441-0, 10/04005-9)

BORGES, LEANDRO; DERMARGOS, ALEXANDRE; GRAY, STUART; BARROS SILVA, MAYSA B.; SANTOS, VINICIUS; PITHON-CURI, TANIA CRISTINA; GORJAO, RENATA; HATANAKA, ELAINE. Neutrophil Migration and Adhesion Molecule Expression after Acute High-Intensity Street Dance Exercise. JOURNAL OF IMMUNOLOGY RESEARCH, 2018. (11/21441-0, 14/21185-1)

SOARES AMORIM, MARIA GUADALUPE; DE OLIVEIRA, MAURICIO DIAS; SOARES, DAIANE SANTOS; BORGES, LEANDRO DA SILVA; DERMARGOS, ALEXANDRE; HATANAKA, ELAINE. Effects of exergaming on cardiovascular risk factors and adipokine levels in women. Journal of Physiological Sciences, v. 68, n. 5, p. 671-678, SEP 2018. (11/21441-0, 14/03947-1)

BORGES, LEANDRO; GORJAO, RENATA; GRAY, STUART R.; MARTINS, THAIS REIS; SANTOS, VINICIUS CONEGLIAN; MOMESSO, CESAR MIGUEL; PITHON-CURI, TANIA CRISTINA; HATANAKA, ELAINE. Lymphocyte activation after a high-intensity street dance class. PLoS One, v. 15, n. 9, p. 9-pg., 2020-09-21. (14/21185-1, 11/21441-0)

BORGES, LEANDRO; DERMARGOS, ALEXANDRE; GRAY, STUART; BARROS SILVA, MAYSA B.; SANTOS, VINICIUS; PITHON-CURI, TANIA CRISTINA; GORJAO, RENATA; HATANAKA, ELAINE. Neutrophil Migration and Adhesion Molecule Expression after Acute High-Intensity Street Dance Exercise. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2018, p. 6-pg., 2018-01-01. (14/21185-1, 11/21441-0)

BORGES, LEANDRO DA SILVA; BORTOLON, JOSE RICARDO; SANTOS, VINICIUS CONEGLIAN; DE MOURA, NIVALDO RIBEIRO; DERMARGOS, ALEXANDRE; CURY-BOAVENTURA, MARIA FERNANDA; GORJAO, RENATA; PITHON-CURI, TANIA CRISTINA; HATANAKA, ELAINE. Chronic Inflammation and Neutrophil Activation as Possible Causes of Joint Diseases in Ballet Dancers. Mediators of Inflammation, v. 2014, p. 7-pg., 2014-01-01. (10/04005-9, 11/21441-0)

SILVA, JAMILA; BORGES, LEANDRO; WEIMANN, ELEINE; DIAS, BEATRIZ BELMIRO; FARIA, FLAVIO GOMES; SALGADO, BEATRIZ FERREIRA; DE FREITAS, PAULO; PITHON-CURI, TANIA CRISTINA; HATANAKA, ELAINE. A Single Bout of High Heels Dancing Causes an Increase in Circulating Markers of Muscle Tissue Degradation and MMP-3 in Young Healthy Women. TRANSLATIONAL SPORTS MEDICINE, v. 2023, p. 7-pg., 2023-06-01. (11/21441-0, 14/21185-1)