Study of simvastatin effect on femoral bone defects in rabbits

• Grant number: 11/19237-5
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2012
• End date: March 31, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Murched Omar Taha
• Grantee: Murched Omar Taha
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Bone loss due to infectious agents, trauma and surgical procedures have been object of several studies aiming bone formation. Osteogenesis needed to repair bone defects may be influenced by several factors, such as arrays used as bone graft material and drugs with direct or indirect action on bone metabolism. The use of statins, particularly simvastatin, have been part of recent studies, with controversial results. In this study, 24 rabbits which are intended to assess the repair of bone tissue to different concentrations of simvastatin. Two surgical standardized, non-critical (4 mm diameter and 6 mm in depth) bone cavities will be made in each rabbit, one in the right and other in the left femur. Each of the cavities will receive one of the following types of fill: 1- simvastatin 1 (SIN 1): bone cavity filled with absorbable collagen sponge (0.02 g) soaked in 0.5 ml of gel simvastatin (simvastatin 1 mg / 0.5 ml HPMC) and biological collagen membrane. 2 -simvastatin 3 (SIN 3): bone cavity filledwith absorbable collagen sponge (0.02 g) soaked in 0.5 ml of gel simvastatin (simvastatin 3 mg / 0.5 ml HPMC) and biological collagen membrane. 3- Vehicle (VEI): bone cavity filled with absorbable collagen sponge (0.02 g) soaked in 0.5 ml of HPMC gel and biological collagen membrane. 4 - Control (CON): bone cavity is filled only with blood clot and biological collagen membrane. The 24 rabbits will be properly marked, identified and randomly divided into 4 groups of 6 animals each: A, B and C and D according to euthanasias time: Group A (7 days),Group B (14 days), Group C (21 days) and Group D (42 days). The bone cavities with the surrounding tissue will be removed and submited to histological and immunohistochemistry avaluation (Osteocalcin and VEGF antibody). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES CARDIM ROSSELLI, JOSE EDUARDO; FONSECA SOARES MARTINS, DULCE MARIA; MARTINS, JOSE LUIZ; CARDIM MENDES DE OLIVEIRA, CLAUDIA REGINA GOMES; FAGUNDES, DJALMA JOSE; TAHA, MURCHED OMAR. The effect of simvastatin on the regeneration of surgical cavities in the femurs of rabbits. Acta Cirurgica Brasileira, v. 29, n. 2, p. 87-92, FEB 2014. (11/19237-5)