| Grant number: | 12/02514-9 |
| Support Opportunities: | Regular Research Grants |
| Start date: | June 01, 2012 |
| End date: | May 31, 2014 |
| Field of knowledge: | Biological Sciences - Biochemistry - Metabolism and Bioenergetics |
| Principal Investigator: | Nilson Antonio de Assunção |
| Grantee: | Nilson Antonio de Assunção |
| Host Institution: | Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil |
Abstract
This project aims to use bioanalytical tools in order to study the radical acetylation of the proteins. The superoxide anion is produced in oxidative stress and reacts with nitric oxide generating peroxynitrite which attacks the alpha carbon of the diacetyl producing radical species. These radicals are able to acetylate proteins and nitrogenous bases in vitro. The exposure to diacetyl is associated to several illnesses, mostly lung diseases. The non controled radical acetylation may cause changes in protein functions and genetic failure which implies in diseases and mutation. The purpose of this work is to verify the radical acetylation caused by peroxinitrite/diacetyl reaction in vivo and the influence of these changes in the proteomic expression in lung tissue. It will be carried out a study using Wistar mice treated with i) inactive ingredient, ii) peroxynitrite; iii) 13C labeled diacetyl; iv) 13C labeled diacetyl and peroxynitrite. The metabolites and proteins extracted from each group will be analyzed by capillary electrophoresis, two dimensional gel electrophoresis, liquid chromatography, western blot and mass spectrometry in order to separate and identify proteins and the position of their acetylation. From these results we hope to answer some relevant biological questions proposing a non enzymatic mechanism to protein acetylation and how it is related with the proteomic expression in oxidative stress and the exposure of the diacetyl. The answers to these questions may contribute to understand the origin of the several illnesses and it will drive the need to control the use of diacetyl in food. (AU)
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