Implementation and optimization of methods for species authentication of cell lines

• Grant number: 12/04164-5
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2012 - June 30, 2014
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Maria Judite Bittencourt Fernandes
• Grantee: Maria Judite Bittencourt Fernandes
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Isabela Cristina Simoni

Abstract

The authenticity and certification of cell lines used in biomedical research is very important to ensure reliable results and to avoid misidentification and cross-contamination among the cells. The analysis of isoenzyme polymorphism is the standard method in quality control of cell line identification and interspecies contamination and is routinely use by main Biological Resource Centers. Other methods have been used such as cytogenetic, immunological and molecular analysis. Molecular methods targeted to DNA mitochondrial (mtDNA) has been used mainly for identifying or confirming the species of origin of animal cell lines. On the other hand, the short tandem repeat profiling have been developed to authenticate human cell lines detecting intraspecies contamination too. The aim of this work is the deployment in our laboratory of one molecular technique (mtDNA) for species identification of animal cell lines besides an improvement of the electrophoretic analysis of isoenzymes which will be used as control and pattern. It is very important to confirm the identities of cell lines as part of quality control in laboratories that work and supply these cells lines. Our laboratory intends to perform and to available this valuable tool of the authentication of the cell lines. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SHIRLEY B. DE ARAÚJO; GIANCARLO F. PATRICIO; ISABELA C. SIMONI; ELIANA B. RIVAS; MARIA JUDITE B. FERNANDES. Isoenzyme and molecular approach for authenticating and monitoring of animal cell lines. Anais da Academia Brasileira de Ciências, v. 91, n. 3, 2019-10-14. (12/04164-5)