Study of the self-assembly of single stranded DNA on modified metallic surfaces

• Grant number: 12/05734-0
• Support Opportunities: Research Grants - Visiting Researcher Grant - Brazil
• Start date: August 01, 2012
• End date: July 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Sidney José Lima Ribeiro
• Grantee: Sidney José Lima Ribeiro
• Visiting researcher: Frederico Guilherme de Carvalho Cunha
• Visiting researcher institution: Universidade Federal de Sergipe (UFS). Centro de Ciências Exatas e Tecnologia (CCET), Brazil
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The main purpose of this proposal is the study of the adsorption process of single stranded DNA of Leishmania (Vianna) brasiliensis on gold electrodes. As a result of this study we expect to create the necessary conditions for the production of a new detection protocol of Leishmaniosis through the blood serum as well as the determination of the species that caused the contagion. The study will be based on the determination of the self assembly process of the single stranded DNA modified by a thiol group adsorbed on single crystalline gold electrodes using Scanning Tunneling Microscopy. The hybridization process will be monitored through the use of Surface Enhanced Raman Spectroscopy on roughened gold electrodes. (AU)