In vivo analyses of genic expression and mutation in quorum sensing gene AI-2 of an avian Pathogenic Escherichia coli strain

• Grant number: 12/09655-7
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2012
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Wanderley Dias da Silveira
• Grantee: Wanderley Dias da Silveira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Escherichia coli strains that cause diseases outside the intestine are known as extra intestinal pathogenic E. coli (ExPEC) which includes human uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC). Our group has been working, for several years, in the study of APECs, in order to characterize these types of bacteria strains for their biological characteristics related to pathogenicity. Recently, it was demonstrated that different types of extracellular signs regulate gene expression in bacteria. When the sign is produced by the bacteria, the process is called quorum sensing (QS) (Anand e Griffiths, 2003). Pathogenic bacteria, through quorum sensing, can achieve high cell density and produce virulence factors enough to surpass the host defenses, before of the possible immunological system recognition (De Kievit et al., 2001). For all we know, there is no work being realized with APEC strains in Brazil and abroad, where the expression of pathogenicity-related genes are analised in vivo and the regulation function of the quorum sensing system, about the general mechanisms of gene expression, including those related to pathogenicity, is being studied. This context led to this proposal, that aims to study the effects of a gene of AI-2 quorum sensing system in the global gene expression, including those possibly related to pathogenicity of APEC, the changes in biological properties and to study pathogenicity-related genes known to be present in this strain in vivo. The results herein obtained will allowed us to work on with the deletion of specific pathogenicity related genes and verify their function in the pathogenicity mechanisms. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PAIVA, JACQUELINE BOLDRIN; LEITE, JANAINA LUISA; MENDES DA SILVA, LIVIA PILATTI; GALVAO ROJAS, THAIS CABRERA; DE PACE, FERNANDA; CONCEICAO, ROGERIO ARCURI; SPERANDIO, VANESSA; DA SILVEIRA, WANDERLEY DIAS. Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain. Veterinary Microbiology, v. 175, n. 1, p. 123-131, JAN 30 2015. (12/09655-7)

VERMA, RENU; GALVAO ROJAS, THAIS CABRERA; MALUTA, RENATO PARIZ; LEITE, JANAINA LUISA; NAKAZATO, GERSON; DE SILVEIRA, WANDERLEY DIAS. Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro. MICROBIOLOGICAL RESEARCH, v. 214, p. 28-36, 2018. (12/23289-3, 12/09655-7, 12/05073-3, 15/03860-6, 13/09167-5, 14/04860-7)

PILATTI, LIVIA; DE PAIVA, JACQUELINE BOLDRIN; GALVAO ROJAS, THAIS CABRERA; LEITE, JANAINA LUISA; CONCEICAO, ROGERIO ARCURI; NAKAZATO, GERSON; DA SILVEIRA, WANDERLEY DIAS. The virulence factor ychO has a pleiotropic action in an Avian Pathogenic Escherichia coli (APEC) strain. BMC Microbiology, v. 16, MAR 10 2016. (12/04391-1, 12/09655-7)