Study of apoptosis gene expression, celular cyclic, DNA repair and oxidative stress in cells of human lang carcinoma treated with complexes with general formula [Ru(AA)(dppb)(bipy)]PF6

• Grant number: 12/06013-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2012
• End date: July 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Alzir Azevedo Batista
• Grantee: Alzir Azevedo Batista
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Researches have demonstrated that ruthenium complexes present great specificity and selectivity by tumor cells than compounds based on platin. Two ruthenium complexes (NAMI and NAMI-A) completed clinic fase I, both with low level of side effects. Several ruthenium complexes have been synthesized and studied by our research group, in the Department of Chemistry/UFSCar, presenting significant citotoxity against different tumor cell lines. Some of these compounds shown specificity against different types of tumor cells and most of them presented citotoxity higher than that of the cisplanin.Based on promising results already obtained by our research group in the Medicinal Inorganic Chemistry area and having in mind that different compounds with different structures can activate different way of cellular signaling we decided to synthesize compounds containing aminoacids, biological molecules, with general formula [Ru(AA)(dppb)(bipy)]PF6 (AA = aminoacids; dppb = 1,4-bis(difenilfosfine)butane; bipy = 2,2´=bipyridine), and to study genes expression of apoptose cellular cyclic, DNA repair and oxidative stress in carcinoma cells of human lang treated with these complexes. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, FLAVIA DE C.; LIMA, BENEDICTO A. V.; DE LIMA, ALINY P.; PIRES, WANESSA C.; MONTEIRO, THALLITA; MAGALHAES, LORENA F.; COSTA, WANDERSON; GRAMINHA, ANGELICA E.; BATISTA, ALZIR A.; ELLENA, JAVIER; et al. Cis-[RuCl(BzCN)(N-N)(P-P)WF6 complexes: Synthesis and in vitro antitumor activity (BzCN = benzonitrile; N-N=2,2 `-bipyridine; 1,10-phenanthroline; P-P=1,4-bis(diphenylphosphino) butane, 1,2-bis(diphenylphosphino)ethane, or 1,1 `-(diphenylphosphino)ferrocene). Journal of Inorganic Biochemistry, v. 149, p. 91-101, AUG 2015. (12/06013-4)

DOS SANTOS, EDJANE R.; CORREA, RODRIGO S.; RIBEIRO, JULIANA U.; GRAMINHA, ANGELICA E.; ELLENA, JAVIER; SELISTRE-DE-ARAUJO, HELOISA S.; BATISTA, ALZIR A.. Ru(II)/bisphosphine/diimine/amino acid complexes: diastereoisomerism, cytotoxicity, and inhibition of tumor cell adhesion to collagen type I. Journal of Coordination Chemistry, v. 69, n. 23, p. 3518-3530, 2016. (12/06013-4, 13/26559-4)

LIMA, BENEDICTO A. V.; CORREA, RODRIGO S.; GRAMINHA, ANGELICA E.; KUZNETSOV, ALEKSEY; ELLENA, JAVIER; PAVAN, FERNANDO R.; LEITE, CLARICE Q. F.; BATISTA, ALZIR A.. Anti-Mycobacterium tuberculosis and Cytotoxicity Activities of Ruthenium(II)/Bipyridine/Diphosphine/Pyrimidine-2-thiolate Complexes: The Role of the Non-Coordinated N-Atom. Journal of the Brazilian Chemical Society, v. 27, n. 1, p. 30-40, JAN 2016. (12/06013-4, 13/26559-4)

BENEDICTO A. V. LIMA; RODRIGO S. CORRÊA; ANGELICA E. GRAMINHA; ALEKSEY KUZNETSOV; JAVIER ELLENA; FERNANDO R. PAVAN; CLARICE Q. F. LEITE; ALZIR A. BATISTA. Anti-Mycobacterium tuberculosis and Cytotoxicity Activities of Ruthenium(II)/ Bipyridine/Diphosphine/Pyrimidine-2-thiolate Complexes: The Role of the Non- Coordinated N-Atom. Journal of the Brazilian Chemical Society, v. 27, n. 1, p. 30-40, 2016-01-00. (12/06013-4, 13/26559-4)

DOS SANTOS, EDJANE R.; GRAMINHA, ANGELICA E.; SCHULTZ, MARIO S.; CORREIA, ISABEL; SELISTRE-DE-ARAUJO, HELOISA S.; CORREA, RODRIGO S.; ELLENA, JAVIER; LACERDA, ELISANGELA DE PAULA S.; PESSOA, JOAO COSTA; BATISTA, ALZIR A.. Cytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes. Journal of Inorganic Biochemistry, v. 182, p. 48-60, MAY 2018. (12/06013-4)

BAROLLI, JOAO P.; CORREA, RODRIGO S.; MIRANDA, FABIO S.; RIBEIRO, JULIANA U.; BLOCH, JR., CARLOS; ELLENA, JAVIER; MORENO, VIRTUDES; COMINETTI, MARCIA R.; BATISTA, ALZIR A.. Polypyridyl Ruthenium Complexes: Novel DNA-Intercalating Agents against Human Breast Tumor. Journal of the Brazilian Chemical Society, v. 28, n. 10, p. 1879-1889, OCT 2017. (13/21611-8, 12/06013-4)

CORREA, RODRIGO S.; DA SILVA, MONIZE M.; GRAMINHA, ANGELICA E.; MEIRA, CASSIO S.; DOS SANTOS, JAMYLE A. F.; MOREIRA, DIOGO R. M.; SOARES, MILENA B. P.; VON POELHSITZ, GUSTAVO; CASTELLANO, EDUARDO E.; BLOCH, JR., CARLOS; et al. Ruthenium(II) complexes of 1,3-thiazolidine-2-thione: Cytotoxicity against tumor cells and anti-Trypanosoma cruzi activity enhanced upon combination with benznidazole. Journal of Inorganic Biochemistry, v. 156, p. 153-163, MAR 2016. (12/06013-4, 09/08131-1, 13/26559-4)

ALMEIDA, MARCIO A. P.; DO NASCIMENTO, FABIO B.; GRAMINHA, ANGELICA E.; FERREIRA, ANTONIO G.; ELLENA, JAVIER; MELLO, FRANCYELLI M. DOS S.; DE LIMA, ALINY P.; SILVEIRA-LACERDA, ELISANGELA DE P.; BATISTA, ALZIR A.. Structural features and cytotoxic activities of [Ru(AA-H)(dppb)(bipy)]PF6 complexes. Polyhedron, v. 81, p. 735-742, OCT 15 2014. (12/06013-4)

LIMA, ALINY PEREIRA; PEREIRA, FLAVIA CASTRO; PINHEIRO ALMEIDA, MARCIO AURELIO; SANTOS MELLO, FRANCYELLI MARIANA; PIRES, WANESSA CARVALHO; PINTO, THALLITA MONTEIRO; DELELLA, FLAVIA KARINA; FELISBINO, SERGIO LUIS; MORENO, VIRTUDES; BATISTA, ALZIR AZEVEDO; et al. Cytoxicity and Apoptotic Mechanism of Ruthenium(II) Amino Acid Complexes in Sarcoma-180 Tumor Cells. PLoS One, v. 9, n. 10, OCT 17 2014. (12/06013-4)

PEREIRA, FLAVIA DE C.; LIMA, BENEDICTO A. V.; DE LIMA, ALINY P.; PIRES, WANESSA C.; MONTEIRO, THALLITA; MAGALHAES, LORENA F.; COSTA, WANDERSON; GRAMINHA, ANGELICA E.; BATISTA, ALZIR A.; ELLENA, JAVIER; et al. Cis-[RuCl(BzCN)(N-N)(P-P)WF6 complexes: Synthesis and in vitro antitumor activity (BzCN = benzonitrile; N-N=2,2 '-bipyridine; 1,10-phenanthroline; P-P=1,4-bis(diphenylphosphino) butane, 1,2-bis(diphenylphosphino)ethane, or 1,1 '-(diphenylphosphino)ferrocene). Journal of Inorganic Biochemistry, v. 149, p. 11-pg., 2015-08-01. (12/06013-4)