Construction and characterization of anti-FGF-2 scFv from 3F12E7 hybridoma

• Grant number: 12/10623-2
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Jane Zveiter de Moraes
• Grantee: Jane Zveiter de Moraes
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Graciela Conceicao Pignatari ; Sang Won Han

Abstract

Melanoma is a potentially fatal disorder and new options for treatment have been studied. Among them, those that aim to prevent growth of blood vessels that nourish the tumor. Associated with conventional cytotoxic therapies, the anti-angiogenic therapy has already shown its potential and its use was approved for the treatment of some tumors. Among the anti-angiogenic drugs are monoclonal antibodies (MAb) against angiogenic factors. A work being completed, developed by our group (FAPESP process 09/18631-1), shows the MAb against fibroblast growth factor -2 (FGF-2) 3F12E7 successfully inhibiting tumor growth and metastatic melanoma formation. MAb 3F12E7 was produced by immunization with human FGF-2 gene. To continue the project, we propose the construction of a single chain variable fragment scFv (single chain variable fragment) using the hybridoma 3F12E7. The antibodies in the form of scFv are constructed by genetic engineering. Being smaller, the scFv easily infiltrates into solid tumor masses. Besides this advantage over the whole antibody, the scFv, when injected into humans, do not cause immune response against the isotypic and allotypic markers, present in the constant regions of the immunoglobulin chains, which allow its injection for unlimited times. In this project, we propose the construction and characterization of the anti-FGF-2 scFv 3F12E7 for further evaluation of its efficiency in cellular and gene therapy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE AGUIAR, RODRIGO BARBOSA; DA SILVA, TABATA DE ALMEIDA; COSTA, BRUNO ANDRADE; MARCONDES MACHADO, MARCELO FERREIRA; YAMADA, RENATA YOSHIKO; BRAGGION, CAMILA; PEREZ, KATIA REGINA; SILVA MORI, MARCELO ALVES; OLIVEIRA, VITOR; DE MORAES, JANE ZVEITER. Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody. SCIENTIFIC REPORTS, v. 11, n. 1, JAN 14 2021. (12/10623-2)