Evaluation of the effects of different conditioning agents on the radicular surface decontamination: in vitro and in vivo study

• Grant number: 12/01980-6
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Adriana Campos Passanezi Santana
• Grantee: Adriana Campos Passanezi Santana
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of this study is to investigate the effects of reducing the time of application of conditioning agents in root surface decontamination in vitro and in vivo. For the in vitro study, 55 teeth extracted for periodontal reasons will be separated into two fragments, accoding to tooth long axis, resulting in 110 fragments which will be divided into 11 groups according to treatment: Group 1A- scaling and root planning (SRP) + phosphoric acid (PA) for 180 seconds; Group 1B- SRP + PA for 90 seconds; Group 2A - SRP + EDTA for 180 seconds; Group 2B - SRP + EDTA for 90 seconds; Group 3A - SRP + citric acid plus tetracycline (CA+T) for 180 seconds; Group 3B - RAR + [AC+T] for 90 seconds; Group 4A - SRP + citric acid (CA) for 90 seconds; Group 4B - SRP + CA for 180 seconds; Group 5A - SRP + HCl tetracycline (HCl-T) for 90 seconds; Group 5B - SRP + HCl-T for 180 seconds; Control - SRP. Fragments will be investigated by scanning electron microscopy to determine the degree of root surface decontamination according to surface roughness index (SRI), residual calculus index (RCI), loss of dental substance index (LDSI), presence of tissue remnants index (PTRI), and removal of smear layer index (RSLI). The results obtained will be statistically analyzed by Kruskal-Wallis. For the in vivo study, the effects of root conditioning with CA+T for 180 seconds (G180), 90 seconds (G90) or no application (G0) on root coverage by subepithelial connective tissue graft (SCTG) will be investigated in 60 patients, both genders, aged 30-60 years, presenting Miller class I or II recessions. Patients will be randomly assigned for one of the study groups (n=20). Patients will be asked about hipersensitivity before surgery and 7, 14, 30 and 180 days after surgery according to a visual scale of painful symptoms. Patients will be periodontally examined by a single and previously calibrated examiner, unaware of the treatment performed, according to probing depth (PD), clinical attachment level (CAL), marginal tissue recession (R), bleeding on probing (BOP), width of keratinized gingiva (KGW), and plaque index (PlI) at baseline and 30, 90 and 180 after surgery. Intra-group statistical analysis will be performed by unpaired t test for each period of evaluation. Inter-group analysis will be performed by analysis of variance for repeated measures. (AU)