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Evaluation of the effects of different conditioning agents in root surface decontamination: an in vitro study

Grant number: 12/12913-8
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): October 06, 2012
Effective date (End): January 05, 2013
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Adriana Campos Passanezi Santana
Grantee:João Paulo Corrêa Barros
Supervisor abroad: Homayoun Hossein Zadeh
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: University of Southern California (USC), United States  
Associated to the scholarship:11/03161-0 - Evaluation of the effects of different conditioning agents on the radicular surface decontamination: in vitro and in vivo study, BP.MS


The effects of root acid treatment have been investigated since many years. Its properties were correlated to acid type, concentration and time of application. Most of these studies have used citric acid pH 1.0, applied for 3 minutes to obtain root decontamination and stimulus for cementogenesis and osteogenesis. However, some adverse effects can be clinically observed after its use, such as hypersensitivity and pulp necrosis. Thus, the aim of this study is to investigate the effects of reducing the time of application of conditioning agents in root surface decontamination in vitro. For this study, 35 teeth extracted for periodontal reasons will be separated into two fragments, according to tooth long axis, resulting in 70 fragments which will be divided into 7 groups according to treatment: Group 1A- scaling and root planning (SRP) + phosphoric acid (PA) for 180 seconds; Group 1B- SRP + PA for 90 seconds; Group 2A - SRP + EDTA for 180 seconds; Group 2B - SRP + EDTA for 90 seconds; Grupo 3A - SRP + citric acid associated with tetracycline (CA+T) for 180 seconds; Grupo 3B - RAR + [AC+T] for 90 seconds; Control - SRP. Fragments will be investigated by scanning electron microscopy on the University of Southern California, EUA to determine the degree of root surface decontamination according to surface roughness index (SRI), residual calculus index (RCI), loss of dental substance index (LDSI), presence of tissue remnants index (PTRI), and removal of smear layer index (RSLI). The results obtained will be statistically analyzed by Kruskal-Wallis. (AU)

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