Evaluation of the effects of different conditioning agents in root surface decontamination: an in vitro study

• Grant number: 12/12913-8
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: October 06, 2012
• End date: January 05, 2013
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Adriana Campos Passanezi Santana
• Grantee: João Paulo Corrêa Barros
• Supervisor: Homayoun Hossein Zadeh
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University of Southern California (USC), United States
• Associated to the scholarship: 11/03161-0 - Evaluation of the effects of different conditioning agents on the radicular surface decontamination: in vitro and in vivo study, BP.MS

Abstract

The effects of root acid treatment have been investigated since many years. Its properties were correlated to acid type, concentration and time of application. Most of these studies have used citric acid pH 1.0, applied for 3 minutes to obtain root decontamination and stimulus for cementogenesis and osteogenesis. However, some adverse effects can be clinically observed after its use, such as hypersensitivity and pulp necrosis. Thus, the aim of this study is to investigate the effects of reducing the time of application of conditioning agents in root surface decontamination in vitro. For this study, 35 teeth extracted for periodontal reasons will be separated into two fragments, according to tooth long axis, resulting in 70 fragments which will be divided into 7 groups according to treatment: Group 1A- scaling and root planning (SRP) + phosphoric acid (PA) for 180 seconds; Group 1B- SRP + PA for 90 seconds; Group 2A - SRP + EDTA for 180 seconds; Group 2B - SRP + EDTA for 90 seconds; Grupo 3A - SRP + citric acid associated with tetracycline (CA+T) for 180 seconds; Grupo 3B - RAR + [AC+T] for 90 seconds; Control - SRP. Fragments will be investigated by scanning electron microscopy on the University of Southern California, EUA to determine the degree of root surface decontamination according to surface roughness index (SRI), residual calculus index (RCI), loss of dental substance index (LDSI), presence of tissue remnants index (PTRI), and removal of smear layer index (RSLI). The results obtained will be statistically analyzed by Kruskal-Wallis. (AU)