Action of intracanl medication on bacterial biofilm located in apical resorption areas of extracted human teeth

• Grant number: 12/08274-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marcia Carneiro Valera Garakis
• Grantee: Marcia Carneiro Valera Garakis
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The aim of this study is to evaluate the effects of intracanal medication on microbial biofilms located in resorptions of apical external root surface of extracted human teeth. 90 roots of extracted human teeth with apical external root surface will be submitted to standardized instrumentation. The roots will be randomly divided into 6 groups of 15 roots each (n=15).The roots will be exposed to E.faecalis strains for 48 hours to induce microbial biofilm formation in resorptions of apical external root surface. After biofilm formation, intracanal medications will be inserted in each group. In the first group (HC) will be inserted calcium hydroxide with sterile saline solution; in the second group (HC-CHX) will be inserted calcium hydroxide with Chlorhexidine gel 2%; in the third group (HC-GEN) will be inserted calcium hydroxide with glycolic extract of ginger 20%; in the fourth group (CHX) will be inserted only chlorhexidine gel 2%, the fifth group (GEN) will be inserted only glycolic extract of ginger 20% and the sixth group (control group - CC) will be inserted sterile saline solution. Each group will remain with medication in the root canal for fifteen (15) days. After this period the roots will be evaluated by calculating the colony-forming units per surface of the specimen (CFU / ml) and by analysis of Scanning Electron Microscope. The results will be analyzed using statistical tests of ANOVA and Tukey. (AU)