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Action of intracanal medication on biofilm located in areas of apical resorption of extracted human teeth

Grant number: 11/04349-2
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Marcia Carneiro Valera Garakis
Grantee:Maria Tereza Pedrosa de Albuquerque
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The aim of this study will evaluate the effect of intracanal dressing on bacterial biofilms located in resorption areas of external apical root surface. Seventy-five roots of extracted human tooth featuring external apical resorption will be subject to standardized instrumentation. The roots will be randomly divided into 5 groups of 15 roots each (n = 15). The roots will be expose to strains of E. faecalis for 48 hours to induce the formation of bacterial biofilm in the areas of external apical resorption. After biofilm formation, intracanal medications will be inserted in each root. In the first group (HC) will be inserted calcium hydroxide, in the second group (HC-CLX) will be inserted calcium hydroxide with chlorhexidine gel 2% in the third group (CLX) will be inserted only 2% chlorhexidine gel, in the fourth group (HC -GEN) will be inserted calcium hydroxide extract of ginger glycolic 20%, and the fifth group (control group - CC) will be inserted saline solution. Each group will remain with medication inside the root canal for a period of 15 (fifteen) days. At the end of this period the roots will be evaluated through the calculation of colony-forming units per surface of the specimen (CFU / ml) and analysis by Scanning Electron Microscope. The results will be analyzed using statistical tests ANOVA and Tukey.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALBUQUERQUE, Maria Tereza Pedrosa de. Ação de medicações intracanal sobre biofilme microbiano localizado em áreas de reabsorção apical de dentes humanos extraídos. 2012. 100 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia de São José dos Campos São José dos Campos.

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