Pregnancy rate, inflammatory response and uterine blood flow affected by pentoxifylline and semen quality assessed in equines

• Grant number: 12/15736-0
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2012
• End date: September 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Eneiva Carla Carvalho Celeghini
• Grantee: Eneiva Carla Carvalho Celeghini
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Rubens Paes de Arruda

Abstract

The aim of this experiment is to evaluate the effect of the semen extender containing Pentoxifylline on sperm characteristics, the uterine clearance and fertility rate, as well as effect of the artificial insemination with different quality semen on the uterine blood flow and fertility rate. Semen samples will be assessed using a protocol of fluorescent probes combination: PI, FITC-PSA e JC-1 and clustered according to percentage of cells with plasma membrane integrity, intact acrosome and high mitochondrial membrane potencial (PIAIC): high quality (40-60% PIAIC spermatozoa) and low quality (10-30% PIAIC spermatozoa) semen. Twenty four mares will be selected and distributed in 6 groups. On the first cycle, 3 groups will be used: Group C (control): not inseminated (n=8), Group D: uterine infusion only with semen extender without Pentoxifylline (n=8) and Group DP: uterine infusion only with semen extender containing Pentoxifylline (n=8). On the second cycle, 3 other groups will be used: Group SS: semen of high quality plus extend without Pentoxifylline (n=8), Group SP: semen of high quality plus extend containing Pentoxifylline (n=8) and Group B: semen of low quality without pentoxifylline (n=8). Uterine blood flow will be measured by transrectal Doppler sonography color and spectral mode. The uterus will be scanned on 7 pre-determined periods: immediately before ovulation induction (follicle e 35mm) (T-30), immediately before artificial insemination (T0), 2 (T2), 6 (T6), 12 (T12), 24 (T24) and 48 hours (T48) after artificial insemination. Twelve hours after artificial insemination endometrial cytology will be done. Pregnancy diagnosis will be assessed on day 14 after AI. For data analyses SAS program, 9.2 version will be used. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TSUNODA, ROBERTA HARUE; ARRUDA, RUBENS PAES; SERRANO-RECALDE, ELENA CAROLINA; MARTINS OLIVEIRA, BRUNA MARCELE; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; RODRIGUES ALVES, MAIRA BIANCHI; LANCONI, RENATA; NICHI, MARCILIO; CARVALHO CELEGHINI, ENEIVA CARLA. Addition of Pentoxifylline to Skim Milk-Based Extender on Frozen-Thawed Equine Sperm. Journal of Equine Veterinary Science, v. 35, n. 10, p. 823-829, OCT 2015. (12/14927-6, 12/15736-0)

TSUNODA, ROBERTA HARUE; SERRANO-RECALDE, ELENA CAROLINA; DE ARRUDA, RUBENS PAES; MARTINS OLIVEIRA, BRUNA MARCELE; COSTA PINTO, SAMARA CRISTINE; CARVALHO CELEGHINI, ENEIVA CARLA. Effects of pentoxifylline supplementation to semen extender on post-breeding inflammation response assessed by endometrial cytology and vascular perfusion in mares. Animal Reproduction Science, v. 208, SEP 2019. (12/14927-6, 12/15736-0)