MicroRNAs expression in infertile women undergoing in vitro fertilization

• Grant number: 12/16911-0
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2013
• End date: June 30, 2015
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Eduardo Leme Alves da Motta
• Grantee: Eduardo Leme Alves da Motta
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Fernanda de Paula Rodrigues ; Francieli Vigo ; Marcia Riboldi ; Tatiana Carvalho de Souza Bonetti

Abstract

Female reproductive function is coordinated by hypothalamus-pituitary-ovarian axis, in which endocrine, cellular and molecular factors act together to develop a promising environment to follicular development and maturation, and a receptive endometrium for embryo implantation. During those processes, a number of genes are super or sub expressed for controlling the ovulatory cycle. The cumulus cells gene expression directly affect the oocyte maturation and then it development potential. In the endometrium, gene expression modifications can regulate the tissue remodeling and implantation window for endometrium and embryos synchrony. In that perspective, small simple strand RNA molecules which act as post-transcriptional regulators of gene expression, the microRNA (miRNA) seems to be essential. Hence, we aim in this study, to evaluate the miRNA expression of endometrial biopsies, endocervix stretch, cumulus cells and peripheral blood from infertile patients undergoing IVF cycles. The possible alterations in miRNA expressions and consequently the gene expression controlling can contribute to folliculogenesis process understanding, and oocyte quality and embryo implantation in IVF cycles. This approach will allows us to find biomarkers to better oocyte and embryo selection in IVF cycles, and will prospect the treatment protocol changes in order to obtain higher endometrial receptivity and clinical outcomes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIGO, FRANCIELI MARIA; FRAIETTA, RENATO; RODRIGUES, FERNANDA; CARVALHO, V, CRISTINA; BONETTI, TATIANA C. S.; MOTTA, EDUARDO L. A.. Cumulus cell microRNA expression when LH is added to the ovarian stimulation protocol: a pilot study. Reproductive BioMedicine Online, v. 43, n. 6, p. 1070-1077, DEC 2021. (12/16911-0)

RODRIGUES, F. P.; BONETTI, T. C.; DE CARVATHO, C. V.; VIGO, F.; FRAIETTA, R.; VILELLA, F.; SIMON, C.; MOTTA, E.. MIRNA EXPRESSION IN RECEPTIVE VERSUS POST-RECEPTIVE ENDOMETRIUM IN PATIENTS UNDERGOING IVF.. Fertility and Sterility, v. 106, n. 3, p. 1-pg., 2016-09-01. (12/16911-0)