Paper-based point of care testing for detection of Staphylococcus in milk from sheep and cow

• Grant number: 11/18267-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: May 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Lea Chapaval Andri
• Grantee: Lea Chapaval Andri
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil

Abstract

Point-of-care tests(POCT)of bacterial infectious agents provides substantial benefits for diagnosis of diseases, mainly by reducing the time needed to get results. Role-based microfluidic lateral flow biosensors offer low cost and are highly sensitive to POCT. This project aims to manufacture and test of a multiplex immuno-specific biosensor for detection of Staphylococcus aureus and two coagulase negative Staphylococcus species from milk of cows and sheep, respectively, suffering from subclinical mastitis. Gold nanoparticles functionalized with conjugated antibodies are used as signs for the bacterial agents. Will also determined the range of detection of bacteria above nominated. Intended to establish the benefits of POCT because it does not require any preprocessing of the sample and is capable of detecting whole bacterial cells. Detection will be done by changing the color, can be detected visually, and provide a rapid, sensitive, low cost and a quantitative tool for the detection of infectious agents present in the sample from cattle and sheep milk. (AU)