Molecular evolution of regulatory regions of HOX genes associated with the morphology of fish fins, with special emphasis on Chondrichthyes

• Grant number: 12/05391-5
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2013
• End date: June 30, 2015
• Field of knowledge: Biological Sciences - Zoology
• Principal Investigator: Marcelo Rodrigues de Carvalho
• Grantee: Marcelo Rodrigues de Carvalho
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Murilo de Carvalho ; Tiana Kohlsdorf

Abstract

The development of genetic regulatory regions, particularly in the genes expressed during embryo development, seems to be closely related processes resulting in the evolutionary origin of new morphology, since variations in regulatory regions determine changes in patterns of gene expression during the formation specific structures. The proposed research aims to investigate associations between the nucleotide sequence of the regulatory region CsB, which controls the expression of 5' end genes of the HoxD cluster (i.e. Hoxd11 and Hoxd13) during the formation of the bud of paired appendages in vertebrates. The sequences will be analyzed for the presence of both conserved regions and regions with high levels of nucleotide substitution, for subsequent inferences about the presence of binding sites for specific transcription factors. It will be studied condrichthyans species representing most of the morphological variation of fins of the major clades (i.e. Squalomorphi and Galeomorphi) and compared with representatives of major groups of Actinopterygii. The fragments of this regulatory region are obtained from PCR reactions with subsequent cloning and sequencing. For this, we used degenerate primers developed in this project collaborating laboratory (FFCLRP-USP) which had previously been tested successfully in a preliminary study for this proposal. The chosen group aims to show how (if any) the morphological variation of fish fins is resulting of molecular evolution of this regulatory region. This study will also contribute to understanding the evolution of species non-genotypic models in studies of evolutionary developmental biology. (AU)

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, JOAO PAULO C. B.; DE CARVALHO, MARCELO R.. Morphology and phylogenetic significance of the pectoral articular region in elasmobranchs (Chondrichthyes). Zoological Journal of the Linnean Society, v. 175, n. 3, p. 525-568, NOV 2015. (12/02349-8, 12/05391-5, 12/09877-0, 12/22692-9)

FONTENELLE, JOAO PEDRO; DE CARVALHO, MARCELO R.. Systematic revision of the Potamotrygon scobina Garman, 1913 species-complex (Chondrichthyes: Myliobatiformes: Potamotrygonidae), with the description of three new freshwater stingray species from Brazil and comments on their distribution and biogeography. Zootaxa, v. 4310, n. 1, p. 1+, AUG 21 2017. (12/02349-8, 12/19479-1, 12/05391-5, 12/09877-0, 11/03952-7)

SOARES, KARLA D. A.; GOMES, ULISSES L.; DE CARVALHO, MARCELO R.. Taxonomic review of catsharks of the Scyliorhinus haeckelii group, with the description of a new species (Chondrichthyes: Carcharhiniformes: Scyliorhinidae). Zootaxa, v. 4066, n. 5, p. 501-534, JAN 19 2016. (12/05391-5, 12/02349-8, 12/09877-0, 14/20316-5)

EBERT, DAVID A.; HAAS, DIANE L.; DE CARVALHO, MARCELO R.. Tetronarce cowleyi, sp nov., a new species of electric ray from southern Africa (Chondrichthyes: Torpediniformes: Torpedinidae). Zootaxa, v. 3936, n. 2, p. 237-250, MAR 19 2015. (12/05391-5, 12/09877-0)

DE CARVALHO, MARCELO R.; WHITE, WILLIAM T.. Narcine baliensis, a new species of electric ray from southeast Asia (Chondrichthyes: Torpediniformes). Zootaxa, v. 4127, n. 1, p. 149-160, JUN 22 2016. (12/05391-5, 12/02349-8, 12/09877-0)

FONTENELLE, JOAO PEDRO; DA SILVA, JOAO PAULO C. B.; DE CARVALHO, MARCELO R.. Potamotrygon limai, sp nov., a new species of freshwater stingray from the upper Madeira River system, Amazon basin (Chondrichthyes: Potamotrygonidae). Zootaxa, v. 3765, n. 3, p. 249-268, FEB 18 2014. (12/02349-8, 02/06459-0, 12/05391-5, 12/09877-0, 11/03952-7)

CARVALHO, MURILO; BOCKMANN, FLAVIO ALICINO; DE CARVALHO, MARCELO RODRIGUES. Homology of the Fifth Epibranchial and Accessory Elements of the Ceratobranchials among Gnathostomes: Insights from the Development of Ostariophysans. PLoS One, v. 8, n. 4, APR 18 2013. (09/54931-0, 12/02349-8, 12/05391-5, 12/05152-0, 04/09219-6, 12/09877-0)

SHIBUYA, AKEMI; DE CARVALHO, MARCELO R.; ZUANON, JANSEN; TANAKA, SHO. The use of pelvic fins for benthic locomotion during foraging behavior in Potamotrygon motoro (Chondrichthyes: Potamotrygonidae). ZOOLOGIA, v. 32, n. 3, p. 8-pg., 2015-05-01. (12/09877-0, 12/05391-5)

SHIBUYA, AKEMI; DE CARVALHO, MARCELO R.; ZUANON, JANSEN; TANAKA, SHO. The use of pelvic fins for benthic locomotion during foraging behavior in Potamotrygon motoro (Chondrichthyes: Potamotrygonidae). Zoologia, v. 32, n. 3, p. 179-186, MAY-JUN 2015. (12/05391-5, 12/09877-0)

WEIGMANN, SIMON; VAZ, DIEGO F. B.; WHITE, WILLIAM T.; DE CARVALHO, MARCELO R.; THIEL, RALF. Distribution and comments on the morphology of Centroscymnus owstonii Garman, 1906 (Squaliformes: Somniosidae), with focus on its occurrence in the Indian Ocean. MARINE BIODIVERSITY, v. 46, n. 3, p. 641-653, SEP 2016. (13/13137-4, 13/05292-0, 12/07712-3, 12/05391-5, 12/05152-0, 12/09877-0)

DA SILVA, JOAO PAULO C. B.; VAZ, DIEGO F. B.; DE CARVALHO, MARCELO R.. Systematic Implications of the Anterior Pectoral Basals in Squaliform Sharks (Chondrichthyes: Elasmobranchii). Copeia, v. 103, n. 4, p. 874-885, DEC 2015. (13/13137-4, 12/02349-8, 12/07712-3, 12/05391-5, 12/09877-0, 12/22692-9)

JONES, CHRISTIAN M.; HOFFMAYER, ERIC R.; HENDON, JILL M.; QUATTRO, JOSEPH M.; LEWANDOWSKI, JUSTIN; ROBERTS, MARK A.; POULAKIS, GREGG R.; AJEMIAN, MATTHEW J.; DRIGGERS, III, WILLIAM B.; DE CARVALHO, MARCELO R.; et al. Morphological conservation of rays in the genus Rhinoptera (Elasmobranchii, Rhinopteridae) conceals the occurrence of a large batoid, Rhinoptera brasiliensis Muller, in the northern Gulf of Mexico. Zootaxa, v. 4286, n. 4, p. 499-514, JUL 4 2017. (12/05391-5, 12/02349-8, 12/09877-0)

VIANA, SARAH T. DE F.; DE CARVALHO, MARCELO R.. Redescription of Squalus acutipinnis Regan, 1908, a Valid Species of Spiny Dogfish from Southern Africa (Chondrichthyes: Squaliformes: Squalidae). Copeia, v. 104, n. 2, p. 539-553, JUL 2016. (11/18861-7, 13/11621-6, 12/05391-5, 12/09877-0)

DA SILVA, JOAO PAULO C. B.; DE CARVALHO, MARCELO R.. Systematics and morphology of Potamotrygon orbignyi (Castelnau, 1855) and allied forms (Chondrichthyes: Myliobatiformes: Potamotrygonidae). Zootaxa, v. 3982, n. 1, p. 1-82, JUL 8 2015. (12/02349-8, 08/09436-8, 02/06459-0, 05/01299-3, 12/05391-5, 12/09877-0)

VIANA, SARAH T. DE F.; DE CARVALHO, MARCELO R.; GOMES, ULISSES L.. Taxonomy and morphology of species of the genus Squalus Linnaeus, 1758 from the Southwestern Atlantic Ocean (Chondrichthyes: Squaliformes: Squalidae). Zootaxa, v. 4133, n. 1, p. 1-89, JUL 4 2016. (12/05391-5, 12/02349-8, 12/09877-0)

DE CARVALHO, MARCELO R.; ROSA, RICARDO S.; DE ARAUJO, MARIA LUCIA G.. A new species of Neotropical freshwater stingray (Chondrichthyes: Potamotrygonidae) from the Rio Negro, Amazonas, Brazil: the smallest species of Potamotrygon. Zootaxa, v. 4107, n. 4, p. 566-585, MAY 4 2016. (12/05391-5, 12/02349-8, 12/09877-0)

PETEAN, FLAVIA DE FIGUEIREDO; DE CARVALHO, MARCELO R.. Comparative morphology and systematics of the cookiecutter sharks, genus Isistius Gill (1864) (Chondrichthyes: Squaliformes: Dalatiidae). PLoS One, v. 13, n. 8, AUG 20 2018. (12/09877-0, 13/09270-0, 12/07668-4, 12/05391-5, 12/02349-8)

WHITE, WILLIAM T.; VAZ, DIEGO F. B.; HO, HSUAN-CHING; EBERT, DAVID A.; DE CARVALHO, MARCELO R.; CORRIGAN, SHANNON; ROCHEL, ELISABETH; DE CARVALHO, MURILO; TANAKA, SHO; NAYLOR, GAVIN J. P.. Redescription of Scymnodon ichiharai Yano and Tanaka 1984 (Squaliformes: Somniosidae) from the western North Pacific, with comments on the definition of somniosid genera. ICHTHYOLOGICAL RESEARCH, v. 62, n. 2, p. 213-229, JAN 2015. (13/13137-4, 13/05292-0, 12/07712-3, 12/05391-5, 12/05152-0, 12/09877-0)

FONTENELLE, JOAO PEDRO; DE CARVALHO, MARCELO R.. Systematic Implications of Brain Morphology in Potamotrygonidae (Chondrichthyes: Myliobatiformes). Journal of Morphology, v. 277, n. 2, p. 252-263, FEB 2016. (12/05391-5, 12/02349-8, 12/09877-0, 10/01240-7)

DA SILVA, JOAO PAULO C. B.; DE CARVALHO, MARCELO R.. Morphology and phylogenetic significance of the pectoral articular region in elasmobranchs (Chondrichthyes). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 175, n. 3, p. 44-pg., 2015-11-01. (12/02349-8, 12/05391-5, 12/22692-9, 12/09877-0)

DA SILVA, JOAO PAULO C. B.; VAZ, DIEGO F. B.; DE CARVALHO, MARCELO R.. Phylogenetic inferences on the systematics of squaliform sharks based on elasmobranch scapular morphology (Chondrichthyes: Elasmobranchii). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 182, n. 3, p. 614-630, MAR 2018. (13/13137-4, 12/02349-8, 12/07712-3, 12/05391-5, 12/09877-0, 12/22692-9)