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Molecular responses of continuous and intermitent swimming and of exogenous melatonin in rats: cell signalizers of the intermediary metabolism and from the inflammatory and oxidative stress pathways

Grant number: 12/20501-1
Support type:Regular Research Grants
Duration: February 01, 2013 - January 31, 2015
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Claudio Alexandre Gobatto
Grantee:Claudio Alexandre Gobatto
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

Animal models are strictly important for the advance of scientific knowledge and, after two decades of research, our group has mastered the performance evaluation and physical training of rats and mices by the standardization of several protocols of aerobic and anaerobic capacity assessment. Recently, we developed an apparatus of tethered swimming and hydrostatic weighing to determine the propulsion force directly and to individualize the effort intensity by the body density respectively. Furthermore, in the physical training field we investigated the effects of systematic training and models of periodization. With the knowledge and tools acquired over the years, now we enabled the possibility of investigating the cellular mechanisms involved in the lactate metabolism, oxidative stress and inflammatory responses under extremely controlled effort intensity situations. Moreover, we will investigate experimental designs that ensure adequate chronobiological and environmental luminosity conditions by providing wakefulness in the night period to preserve the biological characteristics of the rat. In this context, this study will address: 1) the continuous and intermittent effort effects on protein content and gene expression of MCTs 1 and 4, PGC-1alpha and HIF-1alpha of skeletal muscle fibers type I and II; 2) the melatonin administration responses associated to continuous swimming exercise at maximal aerobic capacity on IKK/NF-kB pathway activity, at absolutely controlled situations from the circadian and effort intensity viewpoint, allowing a better understanding of these variables interaction with inflammatory system and oxidative stress. Our hypothesis is that the effort types proposed alter the expression of proteins of the intermediate metabolism differently and that the exogenous melatonin decreases the inflammatory and oxidative stress responses. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SCARIOT, PEDRO P. M.; MANCHADO-GOBATTO, FULVIA DE BARROS; TORSONI, ADRIANA S.; DOS REIS, IVAN G. M.; BECK, WLADIMIR R.; GOBATTO, CLAUDIO A. Continuous Aerobic Training in Individualized Intensity Avoids Spontaneous Physical Activity Decline and Improves MCT1 Expression in Oxidative Muscle of Swimming Rats. FRONTIERS IN PHYSIOLOGY, v. 7, APR 18 2016. Web of Science Citations: 4.
BECK, W.; GOBATTO, C. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats. ACTA PHYSIOLOGICA HUNGARICA, v. 103, n. 1, p. 112-119, MAR 2016. Web of Science Citations: 2.
BECK, WLADIMIR RAFAEL; BOTEZELLI, JOSE DIEGO; PAULI, JOSE RODRIGO; ROPELLE, EDUARDO ROCHETE; GOBATTO, CLAUDIO ALEXANDRE. Melatonin Has An Ergogenic Effect But Does Not Prevent Inflammation and Damage In Exhaustive Exercise. SCIENTIFIC REPORTS, v. 5, DEC 16 2015. Web of Science Citations: 5.
MENEZES SCARIOT, PEDRO PAULO; MANCHADO-GOBATTO, FULVIA DE BARROS; TORSONI, ADRIANA SOUZA; TORSONI, MARCIO ALBERTO; MASSELLI DOS REIS, IVAN GUSTAVO; BECK, WLADIMIR RAFAEL; GOBATTO, CLAUDIO ALEXANDRE. Wide housing space and chronic exercise enhance physical fitness and adipose tissue morphology in rats. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 40, n. 5, p. 489-492, MAY 2015. Web of Science Citations: 2.
BECK, WLADIMIR R.; CAMPESAN, YURI S.; GOBATTO, CLAUDIO A. Validity and reliability of incremental test to determine the anaerobic threshold in swimming rats. INTERNATIONAL JOURNAL OF APPLIED EXERCISE PHYSIOLOGY, v. 4, n. 1, p. 25-33, APR 2015. Web of Science Citations: 2.
WLADIMIR RAFAEL BECK; LUCAS DANTAS MAIA FORTE; CLAUDIO ALEXANDRE GOBATTO. EFEITOS DO HORÁRIO DO DIA, LUMINOSIDADE AMBIENTAL E EXERCÍCIO FÍSICO SOBRE PARÂMETROS INFLAMATÓRIOS E DE PERFORMANCE EM RATOS NADADORES. Rev. educ. fis. UEM, v. 26, n. 1, p. -, Mar. 2015.

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