Relationship of genotypic characteristics of coagulase-negative Staphylococcus with cure rates after antimicrobial treatment of ovine mastitis

• Grant number: 12/23044-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Luiz Francisco Zafalon
• Grantee: Luiz Francisco Zafalon
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil

Abstract

Mastitis in sheep producing milk and meat is recognized to affect the milk quality of sheep, which causes economic losses due to less weight gain in the lambs and risks related to the loss of glands or death of the animal. Coagulase-negative Staphylococcus (CNS) are among the main responsible for the disease in sheep, and among the measures to control the disease caused by these pathogen, the treatment at the end of lactation may contribute to the prevention and cure of subclinical cases. However, cure rates can be reduced by various causes, such as those related to virulence factors presented by microorganisms. The objectives of this project are identified in the milk of sheep that will be treated by dry therapy: CNS species before and after treatment; to establish in these micro-organisms the presence of genes mecA, icaA, icaC, icaD, Bap, Bhp, sea, seb, sec, sed and tst-1, related to antimicrobial resistance ²-lactam antibiotics, biofilm formation and toxin production, respectively. Cure rates for spontaneous subclinical mastitis in sheep and cure rates after treatment will be measured and related to the presence/absence of virulence factors in CNS. The sheep will be randomly assigned to three experimental groups: G1 (control), G2 (infusion of 100 mg of cloxacillin), G3 (infusion of 100 mg of cloxacillin nanoparticulate) after prior screening by the California Mastitis Test (CMT) and somatic cell counts (SCC). The results will be confirmed using the microbiological culture. The informations acquired with the project execution will collaborate with farmers and technicians to the best knowledge of the epidemiology of disease caused by CNS and subsequent prevention and control. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAFALON, LUIZ F.; CUNHA, MARIA L. R. S.; BRANDAO, HUMBERTO M.; MOSQUEIRA, VANESSA C. F.; SANTANA, RAUL C. M.; BARIONI JUNIOR, WALDOMIRO; MARTINS, KATHERYNE B.; PILON, LUCAS E.. Relação entre genes de fatores de virulência em Staphylococcus spp. coagulase-negativos e a falha do tratamento antimicrobiano da mastite subclínica ovina. Pesquisa Veterinária Brasileira, v. 38, n. 4, p. 579-585, APR 2018. (12/23044-0)

LUIZ FRANCISCO ZAFALON; MARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA; DANILO FLÁVIO MORAES RIBOLI; LUCAS EDUARDO PILON. PERSISTÊNCIA DE STAPHYLOCOCCUS COAGULASE-NEGATIVOS EM GLÂNDULAS MAMÁRIAS DE OVELHAS COM MASTITE SUBCLÍNICA APÓS O TRATAMENTO ANTIMICROBIANO À SECAGEM. Ciênc. anim. bras., v. 18, 2017-11-27. (12/23044-0)

PILON, LUCAS EDUARDO; FRANCISCO ZAFALON, LUIZ; MASCARENHAS SANTANA, RAUL COSTA; APARECIDO FIM, GUILHERME JUNIOR; ZANI MANIERI, FERNANDO; DOS SANTOS LOPES, NAHRYDA SAMARA. Coagulase-negative Staphylococcus species isolated from the mammary gland of sheep with subclinical mastitis. BRAZILIAN JOURNAL OF HYGIENE AND ANIMAL SANITY, v. 8, n. 5, p. 6-pg., 2014-09-01. (12/23044-0)