Basic and applied aspects of the industrial use of yeast

• Grant number: 05/01498-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2006
• End date: December 31, 2008
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Cecilia Laluce
• Grantee: Cecilia Laluce
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Our laboratory is requesting funds in order to be able to develop five research subprojects, which present as their common feature the use the same type of microorganism. Nevertheless, they are all of biotechnological interest and they are as follows: -a) Subproject 1: THERMAL STRESS AND CELLULAR HETEROGENEITY OVER A SUCCESSION OF BATCH FERMENTATIONS TO PRODUCE ETHANOL, intending to study the genetic and physiological variations of the yeast population over repetitive fed-batch fermentations; -b) Subproject 2: ACID STRESS AND CELLULAR HETEROGENEITY OVER A SUCCESSION OF BATCH FERMENTATIONS TO PRODUCE ETHANOL with the same goals as those described for the subproject 1 in which temperature variations are replaced by variations in lactic acid initially added at the beginning of each batch fermentation; -c) Subproject 3: IMPROVEMENTS IN THE RESISTANCE OF COMPRESSED YEAST TO HEAT AND DRYNESS, AND RE-HYDRATION OF DRY YEAST CELLS, intending to find out the best conditions of pre-treatment (solutions containing additives and/or temperature variations) for commercial baker's yeast, which can increase the yeast resistance to dryness and storage with minimal reductions in fermentative activity and viability; -d) Subproject 4: THERMOTOLERANT YEASTS APPLIED TO BIOTRANSFORMATIONS IN CHEMISTRY, intending to select yeast strains to excrete high amounts of enzyme of biotechnological interest (proteases, lipases,, esterases, pectinases and pectin lyases), notably in organic catalysis, in order to study their properties and cultivation conditions for maximal excretion of activity in liquid medium: -e) Subproject 5: PHYSICAL CHEMISTRY OF YEAST FLOTATION with the aim to find out flotation additives or additive associations capable of reducing foam volume or changing the affinity of cells to the foam. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GALLARDO, J. C. M.; SOUZA, C. S.; CICARELLI, R. M. B.; OLIVEIRA, K. F.; MORAIS, M. R.; LALUCE, C.. Enrichment of a continuous culture of Saccharomyces cerevisiae with the yeast Issatchenkia orientalis in the production of ethanol at increasing temperatures. Journal of Industrial Microbiology & Biotechnology, v. 38, n. 3, p. 405-414, MAR 2011. (08/56247-6, 05/01498-6)