Periodontal disease progression monitoring through the analysis of molecular markers

• Grant number: 12/15265-7
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Mario Taba Junior
• Grantee: Mario Taba Junior
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Viviane Casagrande Mariguela

Abstract

The aim of the study is to monitor the periodontal effects and characterize the progression of periodontal disease, in active sites with progressive attachment loss, through the analysis of gene expression and proteins present in differents types of samples (saliva, gingival fluid and gingival biopsies) from the same patient/site. Thirty patients will be grouped in one of two groups: control group (C; n = 10) with healthy patients and test group (DP; n = 20) those with diagnosis of periodontitis. The patients will be submitted to the complete periodontal examination, the collection of the samples and to the conventional periodontal treatment. After two months the patients will be reappraised and new samples collection will be carried out. The clinical parameters (biofilm, bleeding on probe, probing depth and clinical attachment) will be used for diagnosis of the clinical status and, based on tolerance method, for the identification of the active sites with progressive attachment loss. The samples will be analyzed through the polymerase chain reaction in real time (Real-Team PCR) and the immunoenzimatic assay (ELISA) to establish the gene expression and proteins of interest related to the inflammation, immune-regulating, destruction of collagen, bone loss and healling. The data will be grouped and submitted to the statistical analysis for verification of differences between the groups C and DP and between the inactive and active sites, and still establish correlation with the molecular profile and between the different samples. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRISTINE D'ALMEIDA BORGES; MILLA SPRONE RICOLDI; MICHEL REIS MESSORA; DANIELA BAZAN PALIOTO; SÉRGIO LUÍS SCOMBATTI DE SOUZA; ARTHUR BELÉM NOVAES JÚNIOR; MARIO TABA JR. Clinical attachment loss and molecular profile of inflamed sites before treatment. Journal of Applied Oral Science, v. 27, 2019-09-09. (12/15265-7)