Population genomics: a new approach for studies of speciation in insects due to host use applied to the development of sustainable strategies of IPM

• Grant number: 12/16266-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: May 01, 2013
• End date: April 30, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Karina Lucas da Silva-Brandão
• Grantee: Karina Lucas da Silva-Brandão
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Celso Omoto ; Felix A. H. Sperling ; Fernando Luis Cônsoli ; Guilherme Corrêa de Oliveira ; Jose Roberto Trigo ; Marcelo Mendes Brandao
• Associated scholarship(s): 15/24663-4 - Biogeographic and diversification patterns of the butterflies from Highlands of Southeastern Brazil, BP.DR; 14/26212-7 - Exploration of next generation sequencing techniques (NGS) for the identification of molecular markers for monitoring the resistance of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) to insecticides and Bt proteins, BP.DR; 13/10491-1 - Population genomics: a new approach for studies of speciation in insects due to host use applied to the development of sustainable strategies of IPM, BP.JP

Abstract

Ecological differentiation and speciation events can be determined by preferential hosts used by an insect species through its geographical range. In this scenario, the genes involved in physiological and behavioral responses to host plant recognition may be implicated in genetic differentiation and reflect population structuring. Here I propose to identify the genetic and evolutionary mechanisms by which ecological speciation and reproductive isolation occur in populations of an important pest insect in Brazil with host races differentiation, the moth Spodoptera frugiperda. The main questions to be answered are how host plants affect the connectivity among populations and lineages differentiation, and how this information can be employed in the integrated pest management (IPM). Next-generation sequencing (NGS) techniques have facilitated the acquisition of extensive data not only for application to population genetics studies but also for exploring these questions related to ecological adaptation, giving origin to the area of population genomics. Here the use of the NGS techniques RNA-Seq and RAD-Seq are proposed for both mining and characterizing single nucleotide polymophisms in populations of Spodoptera frugiperda, and to identify genes under host divergent natural selection within populations of this species. Based on this, it will be possible to investigate and contrast neutral and adaptive variability into these populations, making the elucidation of the evolutionary mechanism of ecological adaptation to host use and its application to the IPM feasible. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BEZERRA DO NASCIMENTO, ANTONIO ROGERIO; PAVINATO, VITOR ANTONIO CORREA; RODRIGUES, JULIANA GONZALES; SILVA-BRANDAO, KARINA LUCAS; CONSOLI, FERNANDO LUIS; MICHEL, ANDREW; OMOTO, CELSO. here is more than chitin synthase in insect resistance to benzoylureas: molecular markers associated with teflubenzuron resistance in Spodoptera frugiperd. JOURNAL OF PEST SCIENCE, v. 95, n. 1, p. 129-144, JAN 2022. (14/26212-7, 12/16266-7, 16/09159-0)

SILVA-BRANDAO, KARINA LUCAS; PERUCHI, ALINE; SERAPHIM, NOEMY; MURAD, NATALIA FARAJ; CARVALHO, RENATO ASSIS; FARIAS, JULIANO RICARDO; OMOTO, CELSO; CONSOLI, FERNANDO LUIS; FIGUEIRA, ANTONIO; BRANDAO, MARCELO MENDES. Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae). PLoS One, v. 13, n. 5, MAY 22 2018. (11/00417-3, 12/16266-7)

SILVA-BRANDAO, KARINA LUCAS; HORIKOSHI, RENATO JUN; BERNARDI, DANIEL; OMOTO, CELSO; FIGUEIRA, ANTONIO; BRANDAO, MARCELO MENDES. Transcript expression plasticity as a response to alternative larval host plants in the speciation process of corn and rice strains of Spodoptera frugiperda. BMC Genomics, v. 18, OCT 16 2017. (11/00417-3, 12/16266-7)

FREITAS, A. V. L.; RAMOS, R. R.; SILVA-BRANDAO, K. L.; COUTOUNE, N.; MAGALDI, L. M.; PABLOS, J. L.; ROSSER, N.; BROWN, JR., K. S.. A New Subspecies of Heliconius hermathena (Nymphalidae: Heliconiinae) from Southern Amazonia. Neotropical Entomology, v. 48, n. 3, p. 467-475, JUN 2019. (11/50225-3, 12/50260-6, 12/16266-7)

MAGALDI, LUIZA MORAES; KAMINSKI, LUCAS AUGUSTO; SERAPHIM, NOEMY; AZEREDO-ESPIN, ANA MARIA; SILVA-BRANDA, KARINA LUCAS; FREITAS, ANDRE VICTOR LUCCI. Hidden in the wing dots: Disentangling mimetic sister species of butterflies (Riodinidae: Stalachtis) with an integrative approach. ZOOLOGISCHER ANZEIGER, v. 294, p. 92-99, SEP 2021. (11/50225-3, 12/16266-7, 12/50260-6, 13/50297-0, 15/24663-4, 10/51340-8)

MURAD, NATALIA FARAJ; SILVA-BRANDAO, KARINA LUCAS; BRANDAO, MARCELO MENDES. Mechanisms behind polyphagia in a pest insect: Responses of Spodoptera frugiperda (JE Smith) strains to preferential and alternative larval host plants assessed with gene regulatory networks. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, v. 1864, n. 3, MAR 2021. (12/16266-7, 11/00417-3)

BERGAMO, LUANA WALRAVENS; SILVA-BRANDAO, KARINA LUCAS; VICENTINI, RENATO; FRESIA, PABLO; LIMA AZEREDO-ESPIN, ANA MARIA. Genetic Differentiation of a New World Screwworm Fly Population from Uruguay Detected by SNPs, Mitochondrial DNA and Microsatellites in Two Consecutive Years. INSECTS, v. 11, n. 8, AUG 2020. (15/02079-9, 12/16266-7)

SILVA-BRANDAO, KARINA LUCAS; MURAD, NATALIA FARAJ; PERUCHI, ALINE; MARTINS, CARLOS HENRIQUE ZANINI; OMOTO, CELSO; FIGUEIRA, ANTONIO; BRANDAO, MARCELO MENDES; TRIGO, JOSE ROBERTO. Transcriptome differential co-expression reveals distinct molecular response of fall-armyworm strains toDIMBOA. Pest Management Science, v. 77, n. 1, SEP 2020. (11/00417-3, 12/16266-7)

LEAL, CAMILA RABELO OLIVEIRA; JORGE, LEONARDO RE; DE SOUZA, ANETE PEREIRA; LEWINSOHN, THOMAS MICHAEL; SILVA-BRANDAO, KARINA LUCAS. Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification. APPLIED ENTOMOLOGY AND ZOOLOGY, v. 56, n. 1, p. 125-132, FEB 2021. (12/16266-7, 15/10236-7, 16/24821-1)

DO NASCIMENTO, ANTONIO ROGERIO BEZERRA; RODRIGUES, JULIANA GONZALES; KANNO, RUBENS HIDEO; ASSUNCAO E DE AMARAL, FERNANDO SEMMELROTH; MALAQUIAS, JOSE BRUNO; SILVA-BRANDAO, KARINA LUCAS; CONSOLI, FERNANDO LUIS; OMOTO, CELSO. Susceptibility monitoring and comparative gene expression of susceptible and resistant strains of Spodoptera frugiperda to lambda-cyhalothrin and chlorpyrifos. Pest Management Science, v. 79, n. 6, p. 14-pg., 2023-03-01. (14/26212-7, 12/16266-7, 13/07375-0, 19/17215-6, 16/09159-0)

SILVA-BRANDAO, KARINA L.; CIRINO, MARIANA; MAGALDI, LUIZA DE MORAES; GUERATTO, PATRICIA EYNG; MATTOS, RICARDO GABRIEL; FREITAS, ANDRE V. L.. ubspecies limits and hidden Wolbachia diversity in Actinote pellenea butterflie. SYSTEMATICS AND BIODIVERSITY, v. 19, n. 8, AUG 2021. (12/16266-7, 13/50297-0, 11/50225-3, 15/24663-4, 18/19171-3, 12/50260-6)

OLIVEIRA LEAL, CAMILA RABELO; DE SOUZA, ANETE PEREIRA; SILVA-BRANDAO, KARINA LUCAS; LEWINSOHN, THOMAS MICHAEL. ost specialization, genetic variability, and differentiation in three species of Tomoplagia from Brazilian rupestrian grassland. Entomologia Experimentalis et Applicata, v. 170, n. 1, SI, NOV 2021. (16/24821-1, 12/16266-7, 15/10236-7)

MASSARDO, DARLI; VANKUREN, NICHOLAS W.; NALLU, SUMITHA; RAMOS, RENATO R.; RIBEIRO, PEDRO G.; SILVA-BRANDAO, KARINA L.; BRANDAO, MARCELO M.; LION, MARILIA B.; FREITAS, ANDRE V. L.; CARDOSO, MARCIO Z.; et al. The roles of hybridization and habitat fragmentation in the evolution of Brazil's enigmatic longwing butterflies, Heliconius nattereri and H. hermathena. BMC Biology, v. 18, n. 1, JUL 3 2020. (12/50260-6, 12/16266-7, 11/00417-3, 11/50225-3)