Antimicrobial and immunological role of cationic peptides in the prevention of early childhood caries and a proposal for incorporation of these peptides in nanoparticles systems as anticaries agents

• Grant number: 12/19235-5
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Cristiane Duque
• Grantee: Cristiane Duque
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Alberto Carlos Botazzo Delbem ; Denise Madalena Palomari Spolidorio ; Eduardo Maffud Cilli ; Juliano Pelim Pessan ; Marlus Chorilli

Abstract

Early childhood caries (ECC) is a microbial infection that can severely compromises the dentition of young children. The etiology of ECC includes infection with selected bacteria, and dietary and host factors. In the oral cavity, the components of the innate immune system like antimicrobial cationic peptides (AMCP) are responsible for the maintenance of the ecological balance and the lack of opportunistic infections. Studies have demonstrated that children with ECC have low levels of some AMCP when compared to caries free children. The incorporation of fragments of their peptides in delivery devices could be an interesting way to prevent severe forms of caries in immunologically susceptible children. The objectives of this study will be to evaluate: 1) the in vivo influence of levels of putative cariogenic microorganisms and immunological factors on development of ECC; 2) the in vitro antimicrobial action of synthetic AMCP isolated/combined incorporated or not in nanoparticles systems. In part 1, biofilm will be collected from caries-active and caries-free children for putative cariogenic microorganism counts by Real Time-PCR. The quantification of AMCP using ELISA also will be quantified from saliva samples. In part 2, ATCC and clinical strains will be used to evaluate microbiological functions of synthetic AMCP, MIC/MBC and biofilm assays tests will be developed. The same microbiological tests will be performed for nanoparticles systems containing isolated or combined AMCP, besides toxicity tests on epithelial cells. Data will be statistically analyzed considering p d 0.05. This study intends to contribute to evaluation of the antimicrobial and immunological role of AMCP in the caries prevention and introduce a proposal for incorporation of these peptides in nanoparticle systems as anticaries agents. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COLOMBO, NATALIA H.; RIBAS, LAIS F. F.; PEREIRA, JESSE A.; KRELING, PAULA F.; KRESSIRER, CHRISTINE A.; TANNER, ANNE C. R.; DUQUE, CRISTIANE. Antimicrobial peptides in saliva of children with severe early childhood caries. ARCHIVES OF ORAL BIOLOGY, v. 69, p. 40-46, SEP 2016. (13/12167-7, 12/19235-5)

COLOMBO, NATALIA HELENA; PEREIRA, JESSE AUGUSTO; RODRIGUES DA SILVA, MARJULLY EDUARDO; FONSECA RIBAS, LAIS FERNANDA; PARISOTTO, THAIS MANZANO; MATTOS-GRANER, RENATA DE OLIVEIRA; SMITH, DANIEL J.; DUQUE, CRISTIANE. Relationship between the IgA antibody response against Streptococcus mutans GbpB and severity of dental caries in childhood. ARCHIVES OF ORAL BIOLOGY, v. 67, p. 22-27, JUL 2016. (13/12167-7, 12/19235-5)

AIDA, KELLY LIMI; KRELING, PAULA FERNANDA; CAIAFFA, KARINA SAMPAIO; FIORAMONTI CALIXTO, GIOVANA MARIA; CHORILLI, MARLUS; SPOLIDORIO, DENISE M. P.; SANTOS-FILHO, NORIVAL ALVES; CILLI, EDUARDO MAFFUD; DUQUE, CRISTIANE. Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 13, p. 3081-3091, 2018. (13/01565-1, 13/12285-0, 13/07600-3, 12/19235-5)

FIORAMONTI CALIXTO, GIOVANA MARIA; DUQUE, CRISTIANE; AIDA, KELLY LIMI; DOS SANTOS, VANESSA RODRIGUES; MASSUNARI, LOIANE; CHORILLI, MARLUS. Development and characterization of p1025-loaded bioadhesive liquid-crystalline system for the prevention of Streptococcus mutans biofilms. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 13, p. 31-41, 2018. (13/01565-1, 13/12285-0, 12/19235-5)

COLOMBO, NATALIA H.; KRELING, PAULA F.; RIBAS, LAIS F. F.; PEREIRA, JESSE A.; KRESSIRER, CHRISTINE A.; KLEIN, MARLISE I.; TANNER, ANNE C. R.; DUQUE, CRISTIANE. Quantitative assessment of salivary oral bacteria according to the severity of dental caries in childhood. ARCHIVES OF ORAL BIOLOGY, v. 83, p. 282-288, NOV 2017. (13/12167-7, 12/19235-5)

ARGANDONA VALDEZ, REMBERTO MARCELO; DOS SANTOS, VANESSA RODRIGUES; CAIAFFA, KARINA SAMPAIO; DANELON, MARCELLE; ARTHUR, RODRIGO ALEX; NEGRINI, THAIS DE CASSIA; BOTAZZO DELBEM, ALBERTO CARLOS; DUQUE, CRISTIANE. Comparative in vitro investigation of the cariogenic potential of bifidobacteria. ARCHIVES OF ORAL BIOLOGY, v. 71, p. 97-103, NOV 2016. (14/02072-1, 12/19235-5)