Tissue engineering: effect of the combination of cells and Bio-sca on bone defect repair

• Grant number: 12/23879-5
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2013 - April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Adalberto Luiz Rosa
• Grantee: Adalberto Luiz Rosa
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Márcio Mateus Beloti ; Murilo Camuri Crovace ; Oscar Peitl Filho ; Paulo Tambasco de Oliveira

Abstract

Tissue engineering is an interdisciplinary science based on the combination of scaffolds, cells and growth factors aiming at repairing lost or damage tissues. Tissue engineering strategies as therapy for the treatment of bone defects resulting from trauma, infections and pathologic processess are of major intereste in oral and maxillofacial and orthopedic fields. Some preliminar results showed that a porous scaffold derived from Biosilicate®, named Bio-sca, presented a high osteogenic potential making it an attractive biomaterial to be used in bone tissue engineering. Thus, the aims of this study are: 1) in vitro evaluation of mesenchymal stem cells (MSC) and preosteoblasts behavior cultured on Bio-sca discs, and 2) in vivo evaluation of the effect of the combination of MSC and preosteblasts with Bio-sca on rat calvarial bone defects repair. MSC and preosteoblasts will be characterized by the expression of surface markers (CD13, CD29, CD31, CD44, CD73, CD90, CD105 e CD166, CD14, CD34, CD45) assayed by flow cytometry and gene expression of a panel of osteoblast markers alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osterix (OSX), type I collagen (COL I), bone morphogenic protein-2 (BMP-2), bone sialoprotein (BSP), osteopontin (OPN) and osteocalcin (OC) by quantitative PCR. Cells cultured on Bio-sca discs will be assayed for cell proliferation, ALP activity, matrix mineralization formation, Runx2 and ALP protein expressions and the gene expresion of the same panel above. In vivo evaluations will be carried out in rat calvarial bone defects treated with Bio-sca+MSC, Bio-sca+preosteoblasts, Bio-sca and non-treated ones and evaluated by microtomographic and histomorphometric analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRAZ, EMANUELA P.; OLIVEIRA, FABIOLA S.; DE OLIVEIRA, PAULO T.; CROVACE, MURILO C.; PEITL-FILHO, OSCAR; BELOTI, MARCIO M.; ROSA, ADALBERTO L.. Bioactive glass-based surfaces induce differential gene expression profiling of osteoblasts. Journal of Biomedical Materials Research Part A, v. 105, n. 2, p. 419-423, FEB 2017. (14/03036-9, 12/23879-5, 12/23525-9)

FERRAZ, EMANUELA PRADO; FREITAS, GILEADE PEREIRA; CROVACE, MURILO CAMURI; PEITL, OSCAR; ZANOTTO, EDGAR DUTRA; DE OLIVEIRA, PAULO TAMBASCO; BELOTI, MARCIO MATEUS; ROSA, ADALBERTO LUIZ. Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering. Biomedical Materials, v. 12, n. 4, AUG 25 2017. (13/07793-6, 12/23879-5, 12/23525-9)

FERRAZ, E. P.; HASSAN, M. Q.; DE OLIVEIRA, P. T.; CROVACE, M.; PEITL-FILHO, O.; BELOTI, M. M.; ROSA, A. L.. The differential expression of osteogenesis-related microRNAS induced by bioactive glass-ceramic in osteoblasts.. MOLECULAR BIOLOGY OF THE CELL, v. 26, p. 2-pg., 2015-01-01. (14/03036-9, 12/23525-9, 12/23879-5)