Topochemistry, porosity and chemical composition determining successful enzymatic sacarification of sugarcane bagasse

• Grant number: 08/56256-5
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Start date: June 01, 2009
• End date: May 31, 2013
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Agreement: CNPq - Pronex
• Principal Investigator: Adriane Maria Ferreira Milagres
• Grantee: Adriane Maria Ferreira Milagres
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Pesquisadores principais: Andre Luis Ferraz
• Associated research grant(s): 12/21995-8 - 35th Symposium on Biotechnology for Fuels and Chemicals, AR.EXT
• Associated scholarship(s): 12/09940-3 - Effect of pretreated sugarcane bagasse lignin on the enzymatic hydrolysis of cellulose, BP.DR; 12/06749-0 - Topochemistry analysis of sugar cane pretreated with alkaline sulfite, BP.IC; 11/08826-0 - Ultrastructural characterization and enzymatic hydrolysis of chemomechanical pretreated sugar cane and sugar cane bagasse, BP.DR

Abstract

This project proposes the development of a new concept for saccharification of sugarcane bagasse based on the enzymatic hydrolysis of forthcoming plants down regulated on lignin biosynthesis. The hypothesis is that once a sugarcane bagasse with reduced lignin content would be available, the hydrolysis of the entire polysaccharide fraction could be performed at mild conditions. Nowadays, the methods used in the pre-treatment of lignocellulosic materials, which precedes the advantageous enzymatic hydrolysis are quite harsh and inevitably lead to degradation and loss of valuable carbohydrates. Moreover, they are generally energy-intensive and generate undesirable by-products, which significantly add processing costs. To check on this hypothesis, the present proposal plan to prepare sugarcane bagasse samples with progressively reduced lignin contents by using a selective chemical step followed by mechanical fiberizing. These bagasse samples would serve as models to find the desirable characteristics, mainly in terms of lignin content, lignin topochemistry and cell wall porosity, necessary to minimize the harshness or abolish the treatment that precedes the enzymatic hydrolysis of the available polysaccharides, namely hemicellulose and cellulose. A subsequent step will be the evaluation of the plants with decreased lignin contents from studies involving down-regulation of lignin biosynthesis or from hybrids selected for low lignin contents. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIQUEIRA, GERMANO; VARNAI, ANIKO; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Enhancement of cellulose hydrolysis in sugarcane bagasse by the selective removal of lignin with sodium chlorite. APPLIED ENERGY, v. 102, n. SI, p. 399-402, FEB 2013. (08/56256-5)

MASARIN, FERNANDO; NEVES, ALINE S.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. Evaluation of a simple alkaline pretreatment for screening of sugarcane hybrids according to their in vitro digestibility. INDUSTRIAL CROPS AND PRODUCTS, v. 51, p. 390-395, NOV 2013. (11/08826-0, 08/56256-5)

SIQUEIRA, GERMANO; ARANTES, VALDEIR; SADDLER, JACK N.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin. BIOTECHNOLOGY FOR BIOFUELS, v. 10, JUL 11 2017. (12/09940-3, 14/06923-6, 08/56256-5)

VALADARES, FERNANDA; GONCALVES, THIAGO A.; GONCALVES, DAYELLE S. P. O.; SEGATO, FERNANDO; ROMANEL, ELISSON; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; FERRAZ, ANDRE. Exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 23 2016. (14/06923-6, 08/56256-5)

COSTA, THALES H. F.; VEGA-SANCHEZ, MIGUEL E.; MILAGRES, ADRIANE M. F.; SCHELLER, HENRIK V.; FERRAZ, ANDRE. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 4 2016. (14/06923-6, 11/50535-2, 08/56256-5)

MENDES, FERNANDA M.; HEIKKILA, ELINA; FONSECA, MARIANA B.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE; FARDIM, PEDRO. Topochemical characterization of sugar cane pretreated with alkaline sulfite. INDUSTRIAL CROPS AND PRODUCTS, v. 69, p. 60-67, JUL 2015. (11/08826-0, 11/50535-2, 08/56256-5)

MESQUITA, JESSICA FARIA; FERRAZ, ANDRE; AGUIAR, ANDRE. Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. Bioprocess and Biosystems Engineering, v. 39, n. 3, p. 441-448, MAR 2016. (14/06923-6, 08/56256-5)

SIQUEIRA, GERMANO; VARNAI, ANIKO; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Enhancement of cellulose hydrolysis in sugarcane bagasse by the selective removal of lignin with sodium chlorite. APPLIED ENERGY, v. 102, p. 4-pg., 2013-02-01. (08/56256-5)

SPORCK, DANIELE; REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; DEL RIO, JOSE C.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. BIOTECHNOLOGY FOR BIOFUELS, v. 10, DEC 7 2017. (14/06923-6, 08/56256-5)

MACHADO, ANGELA DA SILVA; FERRAZ, ANDRE. Biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation. Bioresource Technology, v. 225, p. 17-22, FEB 2017. (14/06923-6, 08/56256-5)

MENDES, FERNANDA M.; FONSECA, MARIANA B.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Anatomic and Ultrastructural Characteristics of Different Regions of Sugar Cane Internodes Which Affect Their Response to Alkaline Sulfite Pretreatment and Material Recalcitrance. ENERGY & FUELS, v. 30, n. 2, p. 1078-1084, FEB 2016. (14/06923-6, 11/50535-2, 08/56256-5)

COSTA, THALES H. F.; MASARIN, FERNANDO; BONIFACIO, TAINA O.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. The enzymatic recalcitrance of internodes of sugar cane hybrids with contrasting lignin contents. INDUSTRIAL CROPS AND PRODUCTS, v. 51, p. 202-211, NOV 2013. (11/50535-2, 08/56256-5)

MOREIRA, SERGIO; MILAGRES, ADRIANE M. F.; MUSSATTO, SOLANGE I.. Reactive dyes and textile effluent decolorization by a mediator system of salt-tolerant laccase from Peniophora cinerea. Separation and Purification Technology, v. 135, p. 183-189, OCT 15 2014. (08/56256-5)

VARNAI, ANIKO; COSTA, THALES H. F.; FAULDS, CRAIG B.; MILAGRES, ADRIANE M. F.; SIIKA-AHO, MATTI; FERRAZ, ANDRE. Effects of enzymatic removal of plant cell wall acylation (acetylation, p-coumaroylation, and feruloylation) on accessibility of cellulose and xylan in natural (non-pretreated) sugar cane fractions. BIOTECHNOLOGY FOR BIOFUELS, v. 7, OCT 15 2014. (11/50535-2, 08/56256-5)

MENDES, FERNANDA M.; LAURITO, DEBORA F.; BAZZEGGIO, MARIANA; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Enzymatic digestion of alkaline-sulfite pretreated sugar cane bagasse and its correlation with the chemical and structural changes occurring during the pretreatment step. BIOTECHNOLOGY PROGRESS, v. 29, n. 4, p. 890-895, JUL 2013. (11/08826-0, 08/56256-5)

LAURITO-FRIEND, D. F.; MENDES, F. M.; REINOSO, F. M.; FERRAZ, A.; MILAGRES, A. M. F.. Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse. BIOMASS & BIOENERGY, v. 75, p. 65-74, APR 2015. (08/56256-5)

CORTEZ, D. V.; ROBERTO, I. C.; BARBOSA, M. H. P.; MILAGRES, A. M. F.. Evaluation of cellulosic and hemicellulosic hydrolysate fermentability from sugarcane bagasse hybrids with different compositions. BIOMASS CONVERSION AND BIOREFINERY, v. 4, n. 4, p. 6-pg., 2014-12-01. (08/56256-5)

Filed patent(s) as a result of this research project