Herbivory and intracellular transport of proteins

• Grant number: 08/52067-3
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Start date: March 01, 2009
• End date: July 31, 2014
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Márcio de Castro Silva Filho
• Grantee: Márcio de Castro Silva Filho
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Daniel Scherer de Moura
• Associated scholarship(s): 13/12577-0 - Study of the molecular basis of SUGARWIN action in phytopathogenic fungi, BP.DR; 09/15421-6 - Deciphering the Spodoptera frugiperda adaptative mechanisms to plant proteinase inhibitors, BP.DR

Abstract

In our lab we are interested in two major research areas: 1) intracellular protein trafficking and, 2) plant-herbivore interactions. These topics have been successfully established over the years and we've obtained international recognition for our research. Our contribution to these fields can be seen in international peer-reviewed journals and also as a referee to world-class journals and national international funding agencies. A Thematic Project that will be merged to the new BIOEN project, based on Plant-herbivore interactions, currently funds intracellular protein trafficking work. Thus, all the aims of the intracellular protein translocation project will be kept but will not be related to the BIOEN initiative. Plant-herbivore interaction is a dynamic system, subjected to continual variation and change. Plants developed different mechanisms to reduce insect attack, inc1uding specific responses that activate different metabolic pathways that considerably alter their chemical and physical aspects. On the other hand, insects developed several strategies to overcome plant defense barriers, allowing them to feed, grow and reproduce on their host plants. This project tries to address important aspects of this complex interaction between sugarcane and herbivore insects, by using different approaches, including: 1) characterization of sugarcane response to major insect pests; 2) understanding the molecular adaptive mechanisms of sugarcane pests; 3) high-throughput gene expression analyses of sugarcane proteinase inhibitors (PI) and serine-proteinases (SP) in response to herbivory by the sugarcane borer (Diatraea saccharalis); 4) subcellular localization of PIs and SPs; 5) characterization of the pool of the giant borer Telchin licus (Drury) digestive proteinases aiming to block them with the use of proteinase inhibitors, and 6) use of Physcomitrella patens as a model for understanding plant response to wounding and other organisms. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, RENATA O.; VIA, ALLEGRA; BRANDAO, MARCELO M.; TRAMONTANO, ANNA; SILVA-FILHO, MARCIO C.. Digestive peptidase evolution in holometabolous insects led to a divergent group of enzymes in Lepidoptera. Insect Biochemistry and Molecular Biology, v. 58, p. 1-11, MAR 2015. (12/03040-0, 11/00417-3, 10/17110-5, 08/52067-3)

SOUZA, THAIS P.; DIAS, RENATA O.; CASTELHANO, ELAINE C.; BRANDAO, MARCELO M.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C.. Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 196, p. 67-73, JUN-JUL 2016. (11/00417-3, 12/03040-0, 08/52067-3, 09/15421-6, 10/17110-5, 08/57908-6)

RODRIGUES, RICARDO A. O.; SILVA-FILHO, MARCIO C.; CLINE, KENNETH. FtsH2 and FtsH5: two homologous subunits use different integration mechanisms leading to the same thylakoid multimeric complex. Plant Journal, v. 65, n. 4, p. 600-609, FEB 2011. (08/52067-3)

BRANDAO, MARCELO M.; SILVA-FILHO, MARCIO C.. Evolutionary History of Arabidopsis thaliana Aminoacyl-tRNA Synthetase Dual-Targeted Proteins. Molecular Biology and Evolution, v. 28, n. 1, p. 79-85, JAN 2011. (05/54618-9, 08/52067-3)

FRANCO, FLAVIA P.; SANTIAGO, ADELITA C.; HENRIQUE-SILVA, FLAVIO; DE CASTRO, PATRICIA ALVES; GOLDMAN, GUSTAVO H.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C.. The Sugarcane Defense Protein SUGARWIN2 Causes Cell Death in Colletotrichum falcatum but Not in Non-Pathogenic Fungi. PLoS One, v. 9, n. 3, MAR 7 2014. (08/57908-6, 08/52067-3)

MEDEIROS, ANE H.; FRANCO, FLAVIA P.; MATOS, JULIANA L.; DE CASTRO, PATRICIA A.; SANTOS-SILVA, LUDIER K.; HENRIQUE-SILVA, FLAVIO; GOLDMAN, GUSTAVO H.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C.. Sugarwin: A Sugarcane Insect-Induced Gene with Antipathogenic Activity. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 25, n. 5, p. 613-624, MAY 2012. (08/57908-6, 08/52067-3)

BRANDAO, MARCELO M.; SPOLADORE, LARISSA; FARIA, LUZINETE C. B.; ROCHA, ANDREA S. L.; SILVA-FILHO, MARCIO C.; PALAZZO, JR., REGINALDO. Ancient DNA sequence revealed by error-correcting codes. SCIENTIFIC REPORTS, v. 5, JUL 10 2015. (11/00417-3, 08/52067-3)

MINGOSSI, FABIANA B.; MATOS, JULIANA L.; RIZZATO, ANA PAULA; MEDEIROS, ANE H.; FALCO, MARIA C.; SILVA-FILHO, MARCIO C.; MOURA, DANIEL S.. SacRALF1, a peptide signal from the grass sugarcane (Saccharum spp.), is potentially involved in the regulation of tissue expansion. Plant Molecular Biology, v. 73, n. 3, p. 271-281, June 2010. (08/11109-5, 08/52067-3)

DO CANTO, AMANDA MORATO; CECILIATO, PAULO H. O.; RIBEIRO, BIANCA; ORTIZ MOREA, FAUSTO ANDRES; FRANCO GARCIA, ANTONIO AUGUSTO; SILVA-FILHO, MARCIO C.; MOURA, DANIEL S.. Biological activity of nine recombinant AtRALF peptides: Implications for their perception and function in Arabidopsis. Plant Physiology and Biochemistry, v. 75, p. 45-54, FEB 2014. (08/52067-3)