Molecular analysis of skin microbiota from healthy subjects and patients with dandruff and seborrheic dermatitis

• Grant number: 08/08183-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: May 01, 2010
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciana Campos Paulino
• Grantee: Luciana Campos Paulino
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated scholarship(s): 11/16543-8 - Genetic diversity analysis of Malassezia sp. from human skin microbiota in healthy subjects and patients with dandruff and seborrheic dermatitis., BP.MS

Abstract

Dandruff and seborrheic dermatitis are chronic dermatological diseases that affect more than half of the population of the world. Their etiology has not been elucidated; however, yeasts from Malassezia genus have been associated with the development of the symptoms. These lipophilic organisms are members of the commensal cutaneous microbiota and have also been associated with skin diseases, although their role in dandruff and seborrheic dermatitis remains unknown. It is also unclear why these organisms are pathogenic for some individuals, and are part of the normal microbiota in others. Cutaneous microbiota also includes bacterial species, which establish an ecological equilibrium with Malassezia sp. The aim of this project is to analyze the skin and scalp microbiota from healthy subjects, comparing it to patients with dandruff and seborrheic dermatitis. More specifically, it will study the microbiota in patients with different levels of severity of the conditions, verify whether or not there are species preferentially associated with the development of the symptoms, analyze the effects of shampoos and medications on Malssezia sp. and on the bacterial microbiota, and evaluate the stability of the microbiota over time. Quantitative real time PCR will be used to detect and to quantify four Malassezia species and an uncharacterized phylotype that represents a potential new species; in addition to three bacterial genera. Furthermore, potential genotypic differences between Malassezia strains will be investigated by comparing ribosomal RNA intergenic sequences, as well as lipase gene sequences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAULINO, LUCIANA CAMPOS. New perspectives on dandruff and seborrheic dermatitis: lessons we learned from bacterial and fungal skin microbiota. EUROPEAN JOURNAL OF DERMATOLOGY, v. 27, n. 1, p. 4-7, JUN 2017. (08/08183-9)

SOARES, RENAN CARDOSO; ZANI, MARCELO BERGAMIN; BELINI BAZAN ARRUDA, ANA CAROLINA; FAVARO DE ARRUDA, LUCIA HELENA; PAULINO, LUCIANA CAMPOS. Malassezia Intra-Specific Diversity and Potentially New Species in the Skin Microbiota from Brazilian Healthy Subjects and Seborrheic Dermatitis Patients. PLoS One, v. 10, n. 2, FEB 19 2015. (08/08183-9, 11/16543-8)

PAULINO, LUCIANA CAMPOS. New perspectives on dandruff and seborrheic dermatitis: lessons we learned from bacterial and fungal skin microbiota. EUROPEAN JOURNAL OF DERMATOLOGY, v. 27, p. 4-pg., 2017-06-01. (08/08183-9)