Advanced search
Start date
Betweenand

Analysis of cutaneous microbiota and immune response in seborrheic dermatitis patients and their family members

Grant number: 15/15808-9
Support type:Regular Research Grants
Duration: October 01, 2016 - September 30, 2018
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Luciana Campos Paulino
Grantee:Luciana Campos Paulino
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Assoc. researchers: Humberto Eduardo Cavallin Calanche

Abstract

The human skin harbors a variety of microorganisms, including Bacteria and Fungi. The majority of Fungi inhabiting healthy human skin belongs to the genus Malassezia; however, these organisms have also been associated with skin diseases, including seborrheic dermatitis (SD). SD is a highly prevalent global disease characterized by pruritus and desquamation, especially on the scalp. Its etiology is still poorly understood, and probably the host immune response also plays an important role in the pathogenic process. Bacterial microbiota from healthy skin is highly diverse, but Propionibacterium sp., Corynebacterium sp. and Staphylococcus sp. are among the most prevalent genera. Little is known regarding acquisition, transition, and interactions between fungal and bacterial microbiota. This work aims to characterize the fungal and bacterial skin microbiota in healthy subjects and SD patients, evaluate patterns of transmission between family members and environment, and analyze the local and systemic host immune response. Skin samples from three body sites from healthy subjects and SD patients and their family members will be collected (five families from each group). Samples of their houses and pets will also be studied. Fungal and bacterial communities will be analyzed by Next Generation Sequencing using universal primers targeting ITS1 region and 16S rDNA from Fungi and Bacteria, respectively. Relative gene expression of IFN-³, IL-1±, IL-2 and IL-8 in skin will be analyzed by quantitative real-time PCR, and serum levels of the same cytokines will be determined by ELISA. Characterizing skin microbiota together with local and systemic immune response will provide a new perspective for understanding the pathogenic process of disease whose etiology has not been elucidated. (AU)