Transcriptomic and proteomic approaches in the study of cardiovascular biomarkers: focus on acute myocardial infarction

• Grant number: 06/03487-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2007
• End date: February 28, 2010
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Mario Hiroyuki Hirata
• Grantee: Mario Hiroyuki Hirata
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The main clinical manifestation of atherosclerosis is the acute myocardial infarction (AMI), which is a medical emergency that requires a prompt diagnosis and efficient therapy. The transcriptomic and proteomic approaches are both powerful tools for the study of AMI and may be instrumental to identify news biomarkers involved in the inflammatory and apoptotic process of cardiovascular diseases. The aim of this study is to determine the gene expression (mRNA and protein) pattern in blood cells following an AMI to identify new biomarkers. For our present studies we will select 10 patients with AMI recently submitted to angioplasty and 6 control individuals, all males, age 20-50 years, from the Instituto Dante Pazzanese do Estado de São Paulo, Brazil. Blood samples will be obtained during the first 48h after AMI for biochemical analysis. A global gene expression pattern of circulating mononuclear cells will be determined using DNA microarray chips (CodelinkTM system). The proteomic analysis of plasma will be carried out with using a ProteinChip® Biomarker System, followed by SELDI (Surface Enhanced Laser Desorption/Ionization) mass spectrometry technology for identification and quantification of proteins. Results from this study will enable us to identify changes in gene and protein profile in the plasma, and may be used as a new diagnostic tool for cardiovascular diseases, as well as biomarkers for prevention, therapy control and prognosis of IAM. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILBIGER, VIVIAN NOGUEIRA; LUCHESSI, ANDRE DUCATI; CRESPO HIRATA, ROSARIO DOMINGUEZ; LIMA NETO, LIDIO GONCALVES; PASTORELLI, CARLA PRISINZANO; UEDA, ERIC K. M.; DOS SANTOS, ELIZABETE SILVA; PEREIRA, MARCOS PAULO; RAMOS, RUI; SAMPAIO, MARCELO FERRAZ; et al. Time course proteomic profiling of human myocardial infarction plasma samples: An approach to new biomarker discovery. Clinica Chimica Acta, v. 412, n. 11-12, p. 1086-1093, MAY 12 2011. (06/03487-4)

LUCHESSI, ANDRE DUCATI; SILBIGER, VIVIAN NOGUEIRA; CERDA, ALVARO; CRESPO HIRATA, ROSARIO DOMINGUEZ; CARRACEDO, ANGEL; BRION, MARIA; INIGUEZ, ANDRES; BRAVO, MARISOL; BASTOS, GUILLERMO; MORAIS REGO SOUSA, AMANDA GUERRA; et al. Increased clopidogrel response is associated with ABCC3 expression: A pilot study. Clinica Chimica Acta, v. 413, n. 3-4, p. 417-421, FEB 18 2012. (06/03487-4)