Mycobacterium tuberculosis: genotyping, resistance profile and analysis of mutations from clinical isolates

• Grant number: 09/53292-3
• Support Opportunities: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
• Start date: July 01, 2010
• End date: November 30, 2012
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: CNPq - PPSUS
• Principal Investigator: Clarice Queico Fujimura Leite
• Grantee: Clarice Queico Fujimura Leite
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

This project aims to understand more about TB that affects diverse populations surrounding of São Paulo (São Paulo, Araraquara) and Mato Grosso do Sul (indigenous and non-indigenous) states, evaluated patients with pulmonary tuberculosis treated in their respective Arnbulatories and Health Services. It will be performed: 1) sputum baciloscopy; 2) Mycobacteria isolation through CULTIVO (BACTEC and LI); 3) species identification through biochemical proof and molecular techniques (PCR and PRA); 4) determining resistance profile/sensibility of mycobacteria strains identified as M tuberculosis against isoniazid and rifampicin drugs; 5) determining the drugs MIC against the clinical isolates of M. tuberculosis; 6) to perform SSCP technique of resistant strains: 7) to perform sequencing of resistance genes to evaluate mutations; 8) to perform molecular typing of M. tuberculosis strains through of MIRU and spoligotyping techniques and 9) to correlate the molecular epidemiology results with classical epidemiology data with the assembly of a database. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARRETO SANTOS, ADOLFO CARLOS; GASPARETO, ROSANGELA MARIA; JUNG VIANA, BRUNILDE HELENA; MENDES, NATALIA HELENA; CLAUDIO PANDOLFI, JOSE RODRIGO; CARDOSO, ROSILENE FRESSATTI; SATO, DAISY NAKAMURA; DE MATOS DAVID, SUSANA CORREIA; FERES SAAD, MARIA HELENA; RASTOGI, NALIN; et al. Mycobacterium tuberculosis population structure shift in a 5-year molecular epidemiology surveillance follow-up study in a low endemic agro-industrial setting in Sao Paulo, Brazil. INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY, v. 2, n. 3, p. 10-pg., 2013-09-01. (09/53292-3)