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Mycobacterium tuberculosis: genotyping, resistance profile and analysis of mutations from clinical isolates


This project aims to understand more about TB that affects diverse populations surrounding of São Paulo (São Paulo, Araraquara) and Mato Grosso do Sul (indigenous and non-indigenous) states, evaluated patients with pulmonary tuberculosis treated in their respective Arnbulatories and Health Services. It will be performed: 1) sputum baciloscopy; 2) Mycobacteria isolation through CULTIVO (BACTEC and LI); 3) species identification through biochemical proof and molecular techniques (PCR and PRA); 4) determining resistance profile/sensibility of mycobacteria strains identified as M tuberculosis against isoniazid and rifampicin drugs; 5) determining the drugs MIC against the clinical isolates of M. tuberculosis; 6) to perform SSCP technique of resistant strains: 7) to perform sequencing of resistance genes to evaluate mutations; 8) to perform molecular typing of M. tuberculosis strains through of MIRU and spoligotyping techniques and 9) to correlate the molecular epidemiology results with classical epidemiology data with the assembly of a database. (AU)

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