Molecular epidemiology of multidrug resistant tuberculosis through whole genome sequencing

• Grant number: 15/13333-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2016
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Valdes Roberto Bollela
• Grantee: Valdes Roberto Bollela
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Wilson Araújo da Silva Junior

Abstract

Tuberculosis (TB) is one of the infectious diseases with greater impact in the world. Currently, research in the field of molecular epidemiology of the disease highlights as a major advance, which allow better understanding on transmission, pathogenesis and bacilli resistance. Among the various genotyping methods developed, whole genome sequencing has shown higher discriminatory power and also enables accurate analysis of gene mutations associated with bacilli resistance. The aim of this study is characterize the Mycobacterium tuberculosis clinical isolates from patients of northeastern state of São Paulo, in Brazil and Sofala Province in Mozambique through whole genome sequencing, and to correlate found genotypes with the presence of bacilli resistance and the clinical form of the disease. It is a descriptive and cross-sectional study, that will be performed at the Clinics Hospital, FMRP-USP (sensitivity tests) and at the Molecular Genetics and Bio-informatics Laboratory on the Hematology Regional Center of Ribeirão Preto (whole genome sequencing). Samples of 140 patients diagnosed with TB during the years 2014-2015 will be included (70 from Brazil and 70 from Mozambique). The project was approved by the Ethics and Research Committee of the HCRP and FMRP-USP (944117 - February 1, 2015) and by the National Bioethics Committee for Health of Mozambique (259 / CNBS / 14 - November 3, 2014). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAMBURETE, EVANGELINA INACIO; DIPPENAAR, ANZAAN; CONCEICAO, EMILYN COSTA; FELICIANO, CINARA; DO NASCIMENTO, MARGARIDA MARIA PASSERI; PERONNI, KAMILA CHAGAS; SILVA JR, WILSON ARAUJO; FERRO, JOSEFO JOAO; HARRISON, LEE H.; WARREN, ROBIN MARK; et al. Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique. TUBERCULOSIS, v. 121, MAR 2020. (15/13333-3, 18/07341-1)

LÍVIA MARIA PALA ANSELMO; EMILYN COSTA CONCEIÇÃO; EVANGELINA NAMBURETE; CINARA FELICIANO; SILVANA SPINDOLA; CHRISTOPHE SOLA; VALDES ROBERTO BOLLELA. Mycobacterium tuberculosis spoligotypes and drug-resistant characterization from Beira compared to genotypes circulating in Mozambique. Revista da Sociedade Brasileira de Medicina Tropical, v. 52, 2019-11-25. (15/13333-3)

FELICIANO, CINARA SILVA; BAZZO MENON, LUCAS JOSE; PALA ANSELMO, LIVIA MARIA; DIPPENAAR, ANZAAN; WARREN, ROBIN MARK; SILVA, JR., WILSON ARAUJO; BOLLELA, VALDES ROBERTO. Xpert MTB/RIF performance to diagnose tuberculosis and rifampicin resistance in a reference centre in southern Brazil. ERJ OPEN RESEARCH, v. 5, n. 3, JUL 1 2019. (15/13333-3)

FELICIANO, CINARA SILVA; NAMBURETE, EVANGELINA INACIO; PLACA, JESSICA RODRIGUES; PERONNI, KAMILA; DIPPENAAR, ANZAAN; WARREN, ROBIN MARK; SILVA, JR., WILSON ARAUJO; BOLLELA, VALDES ROBERTO. Accuracy of whole genome sequencing versus phenotypic (MGIT) and commercial molecular tests for detection of drug-resistant Mycobacterium tuberculosis isolated from patients in Brazil and Mozambique. TUBERCULOSIS, v. 110, p. 59-67, MAY 2018. (15/13333-3)