Glucose and glutamine in cell proliferation and viability

• Grant number: 13/06877-1
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2013
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Monica Talarico Duailibi
• Grantee: Monica Talarico Duailibi
• Host Institution: Centro de Terapia Celular e Molecular. Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Silvio Eduardo Duailibi

Abstract

The anatomical and functional integrity recovery of injured tissues guarantee the survival furthermore, extensive losses of treatment are challenging. Tissue Engineering is an alternative to creating new therapies using biological principles of engineering techniques, which aims the production of human organs and parts replacement in vitro. A large number of viable cells are necessary to organ construction and its obtained from biopsy tissue samples that are expanded in laboratory using cell culture techniques. The in vitro cell expansion depends on atmospheric control conditions, temperature and nutrition. Nutrition is essential for cell expansion, structures and functions maintenance. The main supplements in culture are glucose and glutamine and its control is essential to preserve the viable cells. Objective: To characterize the glucose and glutamine effect on proliferation and cell viability. Methods: The cells are obtained from non-mineralized third molar tissues and cultured in the laboratory. The samples are exposed to a nutritional deficit by animal serum absence in culture medium then nourished by four different culture media, with presence or absence of glucose and glutamine alone or in combination in different concentrations. Apoptosis, proliferation, viability and integrity of cellular structures will be investigated. The Friedman and Wilcoxon tests are used as a statistical analysis, and the confidence interval of 5% is considered significant. The experiments are conducted in 5 to 10 days. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PENNA, VANESSA; LIPAY, MONICA V. N.; DUAILIBI, MONICA T.; DUAILIBI, SILVIO E.. The likely role of proteolytic enzymes in unwanted differentiation of stem cells in culture. FUTURE SCIENCE OA, v. 1, n. 3, NOV 2015. (13/00288-4, 13/06877-1)

MACHADO, NATASHA; DUAILIBI, SILVIO EDUARDO; DOS SANTOS, JENNIFER ADRIANE; PENNA, VANESSA; FERREIRA, LYDIA MASAKO; DUAILIBI, MONICA TALARICO. Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation. Acta Cirurgica Brasileira, v. 29, n. 10, p. 658-666, OCT 2014. (13/06877-1)