Evaluation of different concentrations of selenium in sheep reproduction

• Grant number: 12/50277-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2013
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Eunice Oba
• Grantee: Eunice Oba
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This research project aims to investigate the effects of supplementation with different concentrations of Se and interaction between this with Zn, Pb and Mn on reproduction and production of Santa Inês sheep and Dorper. Will be used 30 sheep in pens managed in intensive system which will be divided into five experimental groups (06 animals / group and three animals per breed): control group (GC1) with supplementation of mineral mixture without addition of selenium and supplemented with other same mineral mixture containing 0.2 (G2), 4 (G3), 12 (G4) and 20 (G5) mg Se / kg of mixture. The trial period will be one year and every 49 days will be collected blood samples by puncturing the jugular vein, semen samples by electroejaculation and feces from the rectum, for each experiment will be an adjustment period of 14 days. After harvesting the semen will be analyzed: turbulence, motility, vigor, concentration and morphology, to assess plasma membrane integrity, acrosomal and mitochondrial association is used fluorescent probes and sperm DNA integrity test will be analyzed by the comet, the fructose and citric acid by the technique of Pereira & Janine; Saffran & Densted respectively. Sodium, potassium and calcium will be analyzed by using ion selective. The concentrations of triglycerides, total cholesterol, LDL and HDL in serum will be determined by enzymatic colorimetric method, levels of zinc and manganese in serum, semen, feces and food will be determined by the technique of Atomic Absorption and Flame of selenium and lead by Atomic Absorption Graphite Furnace, moreover, analyze the concentrations of testosterone, progesterone and insulin in serum by commercial radioimmunoassay kits in the solid phase. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA CANUTO, LUCAS EMANUEL; TEIXEIRA MARTINI SEGABINAZZI, LORENZO GARRIDO; BRAGA DE ARAUJO, ENDRIGO ADONIS; MERCES CHAVES SILVA, LUIS FERNANDO; DE OLIVEIRA, SIDNEI NUNES; DALANEZI, FELIPE MORALES; DELL'AQUA JUNIOR, JOSE ANTONIO; PAPA, FREDERICO OZANAM; OBA, EUNICE. ffect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm qualit. SEMINA-CIENCIAS AGRARIAS, v. 43, n. 2, p. 841-854, MAR-APR 2022. (12/50277-6)

D.C. SILVA; V.M. CODOGNOTO; M. PIAGENTINI; A. DANTAS; G.C. SOUSA; L.S. SILVA; E.R. SOUZA; R.A. ALMEIDA FILHO; R. DENADAI; E. OBA. [Avaliação de componentes bioquímicos e eletrolíticos do sêmen de ovinos suplementados com diferentes concentrações de selênio e sua correlação com a qualidade espermática]. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 74, n. 6, p. 1065-1071, 2023-01-06. (12/50277-6)