Bioactive fraction of Melipona scutellaris geopropolis on Streptococcus mutans biofilm

• Grant number: 13/10879-0
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: August 01, 2013
• End date: January 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Pedro Luiz Rosalen
• Grantee: Pedro Luiz Rosalen
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The aim of this study was to evaluate the influence of the bioactive non-polar fraction of geopropolis on Streptococcus mutans biofilm. The ethanolic extract of Melipona scutellaris geopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF on S. mutans UA159 biofilms generated on saliva-coated hydroxyapatite disks were analyzed by inhibition of formation, killing assay and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 ¼g/mL and 400 ¼g/mL caused 38 % and 53 % reduction in the biomass of biofilm respectively, when compared to vehicle control (p<0.05) subsequently observed at SEM images and this reduction was noticed in the amounts of extracellular alkali-soluble glucans (from 820+82.4 to 352.2+62.1 ¼g, p<0.05), intracellular iodophilic polysaccharides (from 829.9+44.9 to52.4+5.7 ¼g, p<0.05) and proteins (from 1.1+0,2 to 0,48+0,1 ¼g, p<0.05). In addition, the S. mutans viability (killing assay) and acid production by glycolytic pH-drop were not affected (p>0.05). In conclusion, the bioactive HF of geopropolis was promising to control the S. mutans biofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA CUNHA, MARCOS GUILHERME; FRANCHIN, MARCELO; DE CARVALHO GALVAO, LIVIA CAMARA; BUENO-SILVA, BRUNO; IKEGAKI, MASAHARU; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm. Evidence-based Complementary and Alternative Medicine, v. 2013, p. 7-pg., 2013-01-01. (13/10879-0, 09/12354-6, 10/20214-7)

DA CUNHA, MARCOS GUILHERME; FRANCHIN, MARCELO; DE CARVALHO GALVAO, LIVIA CAMARA; BUENO-SILVA, BRUNO; IKEGAKI, MASAHARU; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm. Evidence-based Complementary and Alternative Medicine, 2013. (10/20214-7, 09/12354-6, 13/10879-0)